Document Detail

Determination of benidipine in human plasma using liquid chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  15135106     Owner:  NLM     Status:  MEDLINE    
We developed a method for determining benidipine, a dihydropyridine analogue calcium-channel blocker, in plasma using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Benidipine and benidipine-d5, an internal standard, were extracted from plasma using diethyl ether in the presence of 5M NaOH. After drying the organic layer, the residue was reconstituted in acetonitrile and injected onto a reversed-phase C18 column. The isocratic mobile phase (acetonitrile-5mM ammonium acetate, 90:10, v/v) was eluted at 0.2 ml/min. The ion transitions monitored in multiple reaction-monitoring mode were m/z 506-174 for benidipine and m/z 511-179 for the internal standard. The coefficient of variation of the assay precision was less than 13%, and the accuracy exceeded 92%, except at the limit of quantification, 0.05 ng/ml with 1ml of plasma, when it was 85%. This method was used to measure the benidipine concentration in plasma from healthy subjects after a single 4-mg oral dose of benidipine. This method is a very simple, sensitive, and accurate way to determine the plasma benidipine concentration.
Wonku Kang; Hwi-Yeol Yun; Kwang-Hyeon Liu; Kwang-Il Kwon; Jae-Gook Shin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  805     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2005-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  311-4     Citation Subset:  IM    
Department of Pharmacology and Pharmacogenomics Research Center, Inje University College of Medicine, Busan, Republic of Korea.
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MeSH Terms
Area Under Curve
Calcium Channel Blockers / blood*,  pharmacokinetics
Chromatography, Liquid / methods*
Dihydropyridines / blood*,  pharmacokinetics
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods*
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Dihydropyridines; 105979-17-7/benidipine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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