| Determination of aspartase activity in dairy Propionibacterium strains. | |
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MedLine Citation:
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PMID: 21539599 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Propionic acid bacteria (PAB) are very important as starter cultures for dairy industry in the manufacture of Swiss-type cheeses, in which they are involved in the formation of eyes and are responsible for the typical flavour and aroma. These characteristics are mainly due to the classical propionic acid fermentation, but also the conversion of aspartate to fumarate and ammonia by the enzyme aspartase and the subsequent reduction of fumarate to succinate, which occur in dairy Propionibacterium freudenreichii subsp. shermanii and subsp. freudenreichii starter strains. Additionally, it is known that the metabolism of free amino acids may be partly responsible for secondary fermentation and the subsequent split defects in cheese matrix. In the present study, a determination method for aspartase activity was established and a number of dairy propionibacteria belonging to the subsp. shermanii and freudenreichii were screened for this enzyme activity. A wide range of aspartase activity could be found in PAB isolates originating from cheese. The majority, i.e. 70% of the 100 isolates tested, showed very low levels of aspartate activity. |
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Authors:
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L Blasco; M Kahala; T Tupasela; V Joutsjoki |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-3 |
Journal Detail:
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Title: FEMS microbiology letters Volume: - ISSN: 1574-6968 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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MTT Agrifood Research Finland, Biotechnology and Food Research, FI-31600 Jokioinen. Finland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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