| Determination of antiviral nucleoside analogues AM365 and AM188 in perfusate and bile of the isolated perfused rat liver using HPLC. | |
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MedLine Citation:
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PMID: 16078311 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Development, validation and application of an HPLC assay for new antiviral nucleoside analogues AM365 and AM188 in isolated perfused rat liver perfusate and bile were performed. An analytical column (Phenosphere-NEXT, 250 x 4.6 mm, C(18), 4 microm, Phenomenex) was used in tandem with a guard column (4 x 3 mm, C(18), Phenomenex) and operated at 25 degrees C. The mobile phase [methanol:10 mmol/L sodium orthophosphate buffer (pH 7.0), 15:85, v/v] was pumped at 1 mL/min. The signal from a diode array detector was collected from 190 to 300 nm. The chromatogram was processed at 220 and 252 nm for AM365 and AM188, respectively. The HPLC method was validated by six intraday and seven interday runs. Standard curves were linear in the range 0.125-8.00 microg/mL for AM365 and AM188, and the lower limit of quantification for AM365 and AM188 was 0.125 microg/mL. Mean interday precision and accuracy of IPL perfusate quality control samples were within 8.8%, and mean intraday precision and accuracy were within 13.1%. The assay has been successfully used in the study of metabolism and disposition of AM365 in the isolated perfused rat liver. |
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Authors:
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Jiping Wang; Roger L Nation; Allan M Evans; Susan Cox; Jian Li |
Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 20 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-06-19 Revised Date: 2006-06-23 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
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Languages: eng Pagination: 244-50 Citation Subset: IM |
Copyright Information:
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Copyright 2005 John Wiley & Sons, Ltd. |
Affiliation:
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Centre for Pharmaceutical Research, School of Pharmacy and Medical Sciences, University of South Australia, North Terrace, Adelaide. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-HIV Agents / analysis* Bile / chemistry* Chromatography, High Pressure Liquid / methods* Guanosine / analogs & derivatives*, analysis Liver / chemistry* Nucleosides / analysis* Rats Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/2-amino-9-(3- hydroxy-2-hydroxymethylprop-1-yl)purine; 0/9-(3-hydroxy-2-hydroxymethylprop-1-yl)-guanine; 0/Anti-HIV Agents; 0/Nucleosides; 118-00-3/Guanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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