Document Detail


Determination of anti-tumor agent bis(p-fluorobenzyl)trisulfide and its degraded compound in rat blood using reversed phase high-performance liquid chromatography.
MedLine Citation:
PMID:  18479981     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bis(p-fluorobenzyl)trisulfide (BFTS) demonstrated a broad spectrum of anti-proliferative activity and in vivo anti-tumor efficacy in human xenograft mice models. BFTS is rapidly degraded to its major metabolite bis(p-fluorobenzyl)disulfide (BFDS) in blood. In this study, we developed a reliable procedure for stable storage and treatment of blood samples containing BFTS. An HPLC assay method was developed and validated for the quantitative determination of BFTS and BFDS in rat blood. The developed procedure and method have been successfully utilized to study the concentration-time curves of BFTS and BFDS in the blood of rats after vein injection of 12.5 mg kg(-1) BFTS.
Authors:
Liqiang Gu; Liping Li; Zhongjian Chen; Hao Pan; Huidi Jiang; Su Zeng; Xiao Xu; Haoyun An
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2008-04-25
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  868     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-03     Completed Date:  2008-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / blood*
Chromatography, High Pressure Liquid / methods*
Fluorobenzenes / blood*
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Sulfides / blood*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Fluorobenzenes; 0/Sulfides; 0/fluorapacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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