Document Detail


Determination of alpha-fetoprotein by a new paper disc radioimmunoassay of the sandwich type.
MedLine Citation:
PMID:  65334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Determination of alpha-fetoprotein (AFP) in the 1- to 500-mug/litre range has been shown to be of diagnostic value in a variety of clinical settings, in particular, in prenatal diagnosis and the monitoring of pregnancies. A modified paper disc sandwich radioimmunoassay (sandwich RIA) is described which meets the essential criteria for large-scale assaying for AFP. The sandwich RIA is based upon anti-AFP antiserum, which is available commercially, and thus purified AFP is not essential. The sandwich RIA (1) is simple to run and gives results within 24 h; (2) has a comparatively broad range of sensitivity; (3) requires no apparatus other than a gamma-scintillation counter, and (4) has an acceptable level of accuracy. We have used the described sandwich RIA for AFP in routine obstetrical practice for over 2 years. Our experience, encompassing more than 15,000 samples, has been most satisfactory.
Authors:
S G Johansson; B Kjessler; M S Sherman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International archives of allergy and applied immunology     Volume:  52     ISSN:  0020-5915     ISO Abbreviation:  Int. Arch. Allergy Appl. Immunol.     Publication Date:  1976  
Date Detail:
Created Date:  1977-04-25     Completed Date:  1977-04-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404561     Medline TA:  Int Arch Allergy Appl Immunol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  384-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Female
Humans
Radioimmunoassay / methods*
alpha-Fetoproteins / analysis*
Chemical
Reg. No./Substance:
0/alpha-Fetoproteins

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