| Determination of alpha-fetoprotein by a new paper disc radioimmunoassay of the sandwich type. | |
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MedLine Citation:
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PMID: 65334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Determination of alpha-fetoprotein (AFP) in the 1- to 500-mug/litre range has been shown to be of diagnostic value in a variety of clinical settings, in particular, in prenatal diagnosis and the monitoring of pregnancies. A modified paper disc sandwich radioimmunoassay (sandwich RIA) is described which meets the essential criteria for large-scale assaying for AFP. The sandwich RIA is based upon anti-AFP antiserum, which is available commercially, and thus purified AFP is not essential. The sandwich RIA (1) is simple to run and gives results within 24 h; (2) has a comparatively broad range of sensitivity; (3) requires no apparatus other than a gamma-scintillation counter, and (4) has an acceptable level of accuracy. We have used the described sandwich RIA for AFP in routine obstetrical practice for over 2 years. Our experience, encompassing more than 15,000 samples, has been most satisfactory. |
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Authors:
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S G Johansson; B Kjessler; M S Sherman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International archives of allergy and applied immunology Volume: 52 ISSN: 0020-5915 ISO Abbreviation: Int. Arch. Allergy Appl. Immunol. Publication Date: 1976 |
Date Detail:
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Created Date: 1977-04-25 Completed Date: 1977-04-25 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0404561 Medline TA: Int Arch Allergy Appl Immunol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 384-91 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Female Humans Radioimmunoassay / methods* alpha-Fetoproteins / analysis* |
| Chemical | |
Reg. No./Substance:
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0/alpha-Fetoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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