Document Detail

Determination of allura red in some food samples by adsorptive stripping voltammetry.
MedLine Citation:
PMID:  16385987     Owner:  NLM     Status:  MEDLINE    
Square wave (SW) voltammetry was used to explore the adsorption properties of the food additive dye Allura Red on a hanging mercury drop electrode (HMDE). By using the adsorptive stripping voltammetric approach, we developed a sensitive electroanalytical method for the determination of this azo dye. A well-developed voltammetric peak probably related to the cathodic reduction of the azo moiety was obtained in pH 9 Britton-Robinson (B-R) buffer at 613 mV. Cyclic voltammetric studies indicated that the reduction process was irreversible and primarily controlled by adsorption. The adsorptive voltammetric signal was evaluated with respect to various experimental conditions; the optimized values were supporting electrolyte, B-R buffer; pH 11; accumulation time, 180 s; accumulation potential, 0.0 V; scan rate, 900 mV/s; pulse amplitude, 75 mV; and SW frequency, 90 Hz. Adsorptive voltammetric peak current showed a linear response for Allura Red in the concentration range of 2.5 x 10(-8) to 2.0 x 10(-7) mol/L (r = 0.998). The limit of detection was 8.5 x 10(-9) mol/L (4.2 ng/mL), the precision in terms of relative standard deviation was 1.3%, and the mean recovery was 102%. Possible interferences by several substances usually present in food products such as food additive azo dyes (E110, E102), gelatin, natural and artificial sweeteners, preservatives, and antioxidants were also evaluated. The proposed electrochemical procedure was successfully applied to the determination of this food dye in commercially available candy and a soft drink. The results were compared by statistical evaluation with those obtained by a reference spectrophotometric method.
Ahmad H Alghamdi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  88     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2005 Sep-Oct
Date Detail:
Created Date:  2006-01-02     Completed Date:  2006-02-28     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1387-93     Citation Subset:  IM    
King Saud University, College of Science, Department of Chemistry, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
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MeSH Terms
Azo Compounds / analysis*,  chemistry
Food Analysis / methods*
Food Coloring Agents / analysis*
Hydrogen-Ion Concentration
Reference Standards
Sensitivity and Specificity
Time Factors
Reg. No./Substance:
0/Azo Compounds; 0/Food Coloring Agents; 25956-17-6/Allura Red AC Dye

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