Document Detail


Determination of allergenic egg proteins in food by protein-, mass spectrometry-, and DNA-based methods.
MedLine Citation:
PMID:  20480891     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Egg allergy is one of the most common food allergies in both adults and children, and foods including eggs and their byproducts should be declared under food allergen labeling policies in industrial countries. Therefore, to develop and validate a sensitive and specific method to detect hidden egg allergens in foods, we compared immunochemical, DNA-based, and proteomic methods for detecting egg allergens in foods using egg allergen standards such as egg whole protein, egg white protein, egg yolk protein, ovomucoid, ovalbumin, ovotransferrin, lysozyme, and alpha-livetin. Protein-based immunochemical methods, including ELISA as an initial screening quantitative analysis and immunoblotting as a final confirmatory qualitative analysis, were very sensitive and specific in detecting potentially allergenic egg residues in processed foods in trace amounts. In contrast, the proteomics-based, matrix-assisted laser desorption/ionization time-of-flight MS and LC-tandem quadrupole time-of-flight MS methods were not able to detect some egg allergens, such as ovomucoid, because of its nondenaturing property under urea and trypsin. The DNA-based PCR method could not distinguish between egg and chicken meat because it is tissue-nonspecific. In further studies for the feasibility of these immunochemical methods on 100 real raw dietary samples, four food samples without listed egg ingredients produced a positive response by ELISA, but exhibited negative results by immunoblotting.
Authors:
Ji-Yun Lee; Chang Jong Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-19     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  462-77     Citation Subset:  IM    
Affiliation:
Chung-Ang University, College of Pharmacy, Division of Pathophysiology and Pharmacology, 221 Huksuk-Dong, Dongjak-Ku, Seoul 156-756, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickens
Conalbumin / chemistry
DNA / analysis*
Egg Proteins / chemistry
Egg White / chemistry
Egg Yolk / chemistry
Food Analysis / methods*
Food Hypersensitivity / diagnosis,  immunology*
Humans
Mass Spectrometry / methods*
Muramidase / chemistry
Ovalbumin / chemistry
Ovomucin / chemistry
Ovum / metabolism*
Proteins / analysis*
Proteomics / methods
Chemical
Reg. No./Substance:
0/Egg Proteins; 0/Proteins; 1391-06-6/Conalbumin; 37281-36-0/Ovomucin; 9006-59-1/Ovalbumin; 9007-49-2/DNA; 9008-28-0/livetin; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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