| Determination of allergenic egg proteins in food by protein-, mass spectrometry-, and DNA-based methods. | |
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MedLine Citation:
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PMID: 20480891 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Egg allergy is one of the most common food allergies in both adults and children, and foods including eggs and their byproducts should be declared under food allergen labeling policies in industrial countries. Therefore, to develop and validate a sensitive and specific method to detect hidden egg allergens in foods, we compared immunochemical, DNA-based, and proteomic methods for detecting egg allergens in foods using egg allergen standards such as egg whole protein, egg white protein, egg yolk protein, ovomucoid, ovalbumin, ovotransferrin, lysozyme, and alpha-livetin. Protein-based immunochemical methods, including ELISA as an initial screening quantitative analysis and immunoblotting as a final confirmatory qualitative analysis, were very sensitive and specific in detecting potentially allergenic egg residues in processed foods in trace amounts. In contrast, the proteomics-based, matrix-assisted laser desorption/ionization time-of-flight MS and LC-tandem quadrupole time-of-flight MS methods were not able to detect some egg allergens, such as ovomucoid, because of its nondenaturing property under urea and trypsin. The DNA-based PCR method could not distinguish between egg and chicken meat because it is tissue-nonspecific. In further studies for the feasibility of these immunochemical methods on 100 real raw dietary samples, four food samples without listed egg ingredients produced a positive response by ELISA, but exhibited negative results by immunoblotting. |
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Authors:
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Ji-Yun Lee; Chang Jong Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of AOAC International Volume: 93 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2010 Mar-Apr |
Date Detail:
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Created Date: 2010-05-19 Completed Date: 2010-06-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 462-77 Citation Subset: IM |
Affiliation:
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Chung-Ang University, College of Pharmacy, Division of Pathophysiology and Pharmacology, 221 Huksuk-Dong, Dongjak-Ku, Seoul 156-756, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Conalbumin / chemistry DNA / analysis* Egg Proteins / chemistry Egg White / chemistry Egg Yolk / chemistry Food Analysis / methods* Food Hypersensitivity / diagnosis, immunology* Humans Mass Spectrometry / methods* Muramidase / chemistry Ovalbumin / chemistry Ovomucin / chemistry Ovum / metabolism* Proteins / analysis* Proteomics / methods |
| Chemical | |
Reg. No./Substance:
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0/Egg Proteins; 0/Proteins; 1391-06-6/Conalbumin; 37281-36-0/Ovomucin; 9006-59-1/Ovalbumin; 9007-49-2/DNA; 9008-28-0/livetin; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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