Document Detail


Determination of aflatoxins in food by overpressured-layer chromatography.
MedLine Citation:
PMID:  10895927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Legislative measures for monitoring and control of aflatoxin levels in foods and foodstuffs have been introduced in many countries. The aim of the present work was to make developments in the field of aflatoxin analysis, focusing upon the use of overpressured-layer chromatography (OPLC) for quantitative determination. OPLC methods have been developed for the determination of aflatoxins B1, B2, G1, G2 in different foods. These methods are suitable for sample clean-up and separation as well. Using OPLC we could analyze 10 samples simultaneously. The methods were investigated with fish, corn and wheat samples spiked with 2-10 ng/g aflatoxins. Quantitative evaluation of aflatoxins was accomplished by densitometry. Average recoveries from each food were greater than 73%. The OPLC technique seems to be a rapid, reproducible and cost-effective analysis for quantitative determination of aflatoxins in foods.
Authors:
K H Otta; E Papp; B Bagócsi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  882     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-10-26     Completed Date:  2000-10-26     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  11-6     Citation Subset:  IM    
Affiliation:
Department of Chemical Technology and Environmental Chemistry, L. Eötvös University, Budapest, Hungary. kotta@para.chem.elte.hu
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MeSH Terms
Descriptor/Qualifier:
Aflatoxins / analysis*
Chromatography, Liquid / methods*
Food Analysis / methods*
Pressure
Reference Standards
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Aflatoxins

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