| Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection. | |
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MedLine Citation:
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PMID: 8263094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). limits of detection were estimated to be 1200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (microgram/ml) were approximately the same as in sciatic nerve (microgram/g). |
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Authors:
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J H Raymer; C M Sparacino; G R Velez; S Padilla; R C MacPhail; K M Crofton |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 619 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1994-01-26 Completed Date: 1994-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 223-34 Citation Subset: IM |
Affiliation:
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Research Triangle Institute, Research Triangle Park, NC 27709. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylamide Acrylamides / analysis*, blood, pharmacokinetics Animals Chromatography, Gas Gas Chromatography-Mass Spectrometry Half-Life Magnetic Resonance Spectroscopy Male Rats Sciatic Nerve / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Acrylamides; 79-06-1/Acrylamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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