Document Detail

Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.
MedLine Citation:
PMID:  8263094     Owner:  NLM     Status:  MEDLINE    
A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). limits of detection were estimated to be 1200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (microgram/ml) were approximately the same as in sciatic nerve (microgram/g).
J H Raymer; C M Sparacino; G R Velez; S Padilla; R C MacPhail; K M Crofton
Related Documents :
24647154 - Antioxidant activity of rafflesia kerrii flower extract.
7353284 - Two-hour sephadex column method for assay of unconjugated estriol in serum.
10323474 - Determination of benzodiazepines in clinical serum samples: comparative evaluation of r...
6838144 - A rapid procedure for the simultaneous estimation of the anticonvulsant drugs, ethosuxi...
537864 - Triazine herbicide residues in central european streams.
11714344 - Antioxidant properties of methanolic extracts from several ear mushrooms.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  619     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1994-01-26     Completed Date:  1994-01-26     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  223-34     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acrylamides / analysis*,  blood,  pharmacokinetics
Chromatography, Gas
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Sciatic Nerve / chemistry*
Reg. No./Substance:
0/Acrylamides; 20R035KLCI/Acrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Solid-phase extraction and gas chromatographic-mass spectrometric determination after hydrolysis of ...
Next Document:  Gas chromatographic-mass spectrometric screening procedure for the identification of formaldehyde-de...