Document Detail


Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.
MedLine Citation:
PMID:  8263094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). limits of detection were estimated to be 1200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (microgram/ml) were approximately the same as in sciatic nerve (microgram/g).
Authors:
J H Raymer; C M Sparacino; G R Velez; S Padilla; R C MacPhail; K M Crofton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  619     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1994-01-26     Completed Date:  1994-01-26     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  223-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrylamide
Acrylamides / analysis*,  blood,  pharmacokinetics
Animals
Chromatography, Gas
Gas Chromatography-Mass Spectrometry
Half-Life
Magnetic Resonance Spectroscopy
Male
Rats
Sciatic Nerve / chemistry*
Chemical
Reg. No./Substance:
0/Acrylamides; 20R035KLCI/Acrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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