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Determination of Thermally Induced Trans-Fatty Acids in Soybean Oil by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy and Gas Chromatography Analysis.
MedLine Citation:
PMID:  23025355     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The intake of edible oil containing trans-fatty acids has deleterious effects mainly on the cardiovascular system. Thermal processes such as refining and frying cause the formation of trans-fatty acids in edible oil. This study was conducted to investigate the possible formation of trans-fatty acids because of the heat treatment of soybean oil. The types of trans-fatty acids in heated soybean oil are determined by attenuated total reflectance-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry method. The effects of heating temperature on the trans-fatty acids in soybean oil were evaluated using gas chromatography-flame ionization detection analysis. Results show that heat treatment at 240 °C causes the formation of trans-fatty acids in soybean oil and the amount of trans-fatty acids increases with heating time. The only peak observed at 966 cm-1 of the samples indicates the formation of non-conjugated trans isomers in the heated soybean oil. The major types of trans-fatty acids formed were trans-polyunsaturated fatty acids. Significant increases (P < 0.05) in the amounts of two trans-linoleic acids (C18:2-9c,12t and C18:2-9t,12c) and Four trans-linolenic acids (C18:3-9c,12c,15t, C18:3-9t,12c,15c and C18:3-9t,12t,15c/C18:3-9t,12c,15t) in soybean oil heated to temperatures exceeding 200 °C were compared with the control sample. Heating temperature and duration should be considered to reduce the formation of trans-fatty acids during thermal treatment.
Authors:
An Li; Yiming Ha; Feng Wang; Weiming Li; Qingpeng Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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