Document Detail

Determination of sialic acids in milks and milk-based products.
MedLine Citation:
PMID:  20553868     Owner:  NLM     Status:  MEDLINE    
Sialic acids are becoming recognized as important components of milk-based products for infants and young children. As such, many companies now label the sialic acid content of their products. To control the labeling, suitable methods are required for this analysis. The objective of this work was to set up a rapid and sensitive method for the determination of the two most commonly occurring sialic acids, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), using high-performance liquid chromatography (HPLC). The sialic acids were released from their parent oligosaccharides, glycoproteins, or glycolipids by mild acid hydrolysis using formic acid. They were then derivatized using 1,2-diamino-4,5-methylenedioxybenzene (DMB) and subsequently separated on a Zorbax SB-Aq Rapid Resolution column in less than 2 min. The method developed was validated on various milk-based products and ingredients containing sialic acid at levels from 0.3 to 900 mg/100 g. Spiking experiments indicate that the sialic acid recoveries ranged from 87% to 108%. The expanded measurement uncertainty was typically below 15% for Neu5Gc and typically below 10% for Neu5Ac or the sum of the sialic acids, with a few exceptions. The proposed method is fast, specific, and easy to set up for compliance analysis in a routine laboratory.
Véronique Spichtig; Julien Michaud; Sean Austin
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Publication Detail:
Type:  Journal Article     Date:  2010-06-08
Journal Detail:
Title:  Analytical biochemistry     Volume:  405     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-07-27     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-40     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Nestlé Research Centre, Nestec, CH-1000 Lausanne 26, Switzerland.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Milk / chemistry*
Milk, Human / chemistry
N-Acetylneuraminic Acid / analysis*
Neuraminic Acids / analysis
Phenylenediamines / chemistry
Reg. No./Substance:
0/Neuraminic Acids; 0/Phenylenediamines; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid; 38608-07-0/1,2-diamino-4,5-methylenedioxybenzene

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