| Determination of sialic acids in milks and milk-based products. | |
| | |
MedLine Citation:
|
PMID: 20553868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sialic acids are becoming recognized as important components of milk-based products for infants and young children. As such, many companies now label the sialic acid content of their products. To control the labeling, suitable methods are required for this analysis. The objective of this work was to set up a rapid and sensitive method for the determination of the two most commonly occurring sialic acids, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), using high-performance liquid chromatography (HPLC). The sialic acids were released from their parent oligosaccharides, glycoproteins, or glycolipids by mild acid hydrolysis using formic acid. They were then derivatized using 1,2-diamino-4,5-methylenedioxybenzene (DMB) and subsequently separated on a Zorbax SB-Aq Rapid Resolution column in less than 2 min. The method developed was validated on various milk-based products and ingredients containing sialic acid at levels from 0.3 to 900 mg/100 g. Spiking experiments indicate that the sialic acid recoveries ranged from 87% to 108%. The expanded measurement uncertainty was typically below 15% for Neu5Gc and typically below 10% for Neu5Ac or the sum of the sialic acids, with a few exceptions. The proposed method is fast, specific, and easy to set up for compliance analysis in a routine laboratory. |
| | |
Authors:
|
Véronique Spichtig; Julien Michaud; Sean Austin |
Related Documents
:
|
4044028 - Cytolytic effect of polylysine on rabbit polymorphonuclear leukocytes. 1626178 - The utility of lipid-associated sialic acid (lasa or lsa) as a serum marker for maligna... 42648 - Enzymatic properties of neuraminidases from arthrobacter ureafaciens. 814368 - Demonstration of carbohydrate structures in malignant melanoma tyrosinase. 6230988 - In vitro activities of ciprofloxacin, norfloxacin, pipemidic acid, cinoxacin, and nalid... 9872078 - Analysis of hyaluronic acid in human gingival crevicular fluid using high-performance l... |
Publication Detail:
|
Type: Journal Article Date: 2010-06-08 |
Journal Detail:
|
Title: Analytical biochemistry Volume: 405 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-07-27 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 28-40 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Nestlé Research Centre, Nestec, CH-1000 Lausanne 26, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle Chromatography, High Pressure Liquid / methods* Humans Milk / chemistry* Milk, Human / chemistry N-Acetylneuraminic Acid / analysis* Neuraminic Acids / analysis Phenylenediamines / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Neuraminic Acids; 0/Phenylenediamines; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid; 38608-07-0/1,2-diamino-4,5-methylenedioxybenzene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparison of Label-Free Biosensing in Microplate, Microfluidic, and Spot-Based Affinity Capture Ass...
Next Document: A Label-Free Nanoparticle Aggregation Assay for Protein Complex/Aggregate Detection and Study.