| Determination of Oxidative Glucose Metabolism In Vivo in the Young Rat Brain Using Localized Direct-Detected (13)C NMR Spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 21660589 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Determination of oxidative metabolism in the brain using in vivo (13)C NMR spectroscopy ((13)C MRS) typically requires repeated blood sampling throughout the study to measure blood glucose concentration and fractional enrichment (input function). However, drawing blood from small animals, such as young rats, placed deep inside the magnet is technically difficult due to their small total blood volume. In the present study, a custom-built animal holder enabled temporary removal of the animal from the magnet for blood collection, followed by accurate repositioning in the exact presampling position without degradation of B(0) shimming. (13)C label incorporation into glutamate C4 and C3 positions during a 120 min [1,6-(13)C(2)] glucose infusion was determined in 28-day-old rats (n = 4) under α-chloralose sedation using localized, direct-detected in vivo (13)C MRS at 9.4T. The tricarboxylic acid cycle activity rate (V (TCA)) determined using a one-compartment metabolic modeling was 0.67 ± 0.13 μmol/g/min, a value comparable to previous ex vivo studies. This methodology opens the avenue for in vivo measurements of brain metabolic rates using (13)C MRS in small animals. |
| | |
Authors:
|
Kathleen Ennis; Dinesh Kumar Deelchand; Ivan Tkac; Pierre-Gilles Henry; Raghavendra Rao |
Related Documents
:
|
1931469 - Prolonged effect of cgrp in raynaud's patients: a double-blind randomised comparison wi... 7963259 - Effects of neural blockade and general anesthesia on the laser-doppler skin blood flow ... 21987349 - Development of a high-sensitivity bgo well counter for small animal pet studies. 11063089 - Intravascular circulation and distribution of human 51cr-dbbf stroma-free hemoglobin, 5... 15948979 - Dual wavelength (532 and 633 nm) laser doppler imaging of plaque psoriasis. 17016119 - Adequacies of skin puncture for evaluating biochemical and hematological blood paramete... 2277509 - Effect of convection in capillaries on oxygen removal from arterioles in striated muscle. 7653159 - Subtle differences in glycosylation of blood group m and n type glycophorin a detected ... 21658139 - Prevalence of immunoglobulin a deficiency in chinese blood donors and evaluation of ana... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-10 |
Journal Detail:
|
Title: Neurochemical research Volume: - ISSN: 1573-6903 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Pediatrics, Division of Neonatology, University of Minnesota, Mayo Mail Code 39, 420 Delaware Street, SE, Minneapolis, MN, 55455, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Avoidant coping moderates the association between anxiety and patient-rated physical functioning in ...
Next Document: Risperidone in Ultra Low Dose Protects Against Stress in the Rodent Cold Restraint Model by Modulati...