| Determination of N-acetylneuraminic acid by gas chromatography-mass spectrometry with a stable isotope as internal standard. | |
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MedLine Citation:
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PMID: 6625156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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N-Acetylneuraminic acid was determined by gas chromatography-mass spectrometry using selected ion-monitoring technique with N-[2H3]acetylneuraminic acid as an internal standard. M-COOTMS fragments at m/z 624 of trimethylsilyl derivatives of N-acetylneuraminic acid and at m/z 627 of that of the internal standard were used as monitoring ions. The standard curve obtained was linear in the range of over 10(3), and the lower limit for quantitation was estimated to be a few hundred picograms. This method was used to measure total N-acetylneuraminic acid in the plasma of healthy humans and patients with lung cancer. The total N-acetylneuraminic acid level in the plasma was two to three times higher in the patients than in controls. A few hundred nanoliters of plasma was sufficient for the analysis. The mass fragmentogram of plasma gave a good signal/noise ratio, and measurements were very specific, accurate, and reproducible. |
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Authors:
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Y Sugawara; M Iwamori; J Portoukalian; Y Nagai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 132 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1983-11-25 Completed Date: 1983-11-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 147-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Gas Chromatography-Mass Spectrometry Humans Lung Neoplasms / blood* N-Acetylneuraminic Acid Reference Standards Sialic Acids / blood*, standards Trimethylsilyl Compounds |
| Chemical | |
Reg. No./Substance:
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0/Sialic Acids; 0/Trimethylsilyl Compounds; 131-48-6/N-Acetylneuraminic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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