| Determination of N-acetylglucosaminyltransferases III, IV and V in normal and hepatoma tissues of rats. | |
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MedLine Citation:
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PMID: 2145037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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N-Acetylglucosaminyltransferase III, IV and V activities were assayed in various rat tissues and hepatomas using the same fluorescence-labeled sugar chain, GlcNAc beta 1-2Man alpha 1-3-(GlcNAc beta 1-2Man alpha 1-6)Man beta 1-4GlcNAc beta 1-4GlcNAc-2-aminopyridine as a substrate. The N-acetylglucosaminyltransferase III activity toward the substrate is the highest in most rat tissues including primary rat hepatoma. A relatively higher activity for GnT-V is found in small intestine, serum and hepatoma as compared to that of GnT-IV. Some kinetic properties of these enzymes in crude extracts were also determined. |
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Authors:
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A Nishikawa; J Gu; S Fujii; N Taniguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1035 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1990-11-06 Completed Date: 1990-11-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 313-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Osaka University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Sequence Glucosyltransferases / metabolism* Kinetics Liver Neoplasms, Experimental / enzymology* Molecular Sequence Data N-Acetylglucosaminyltransferases* Oligosaccharides / metabolism Organ Specificity Rats Reference Values Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.144/beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; EC 2.4.1.145/alpha-1,3-mannosylglycoprotein beta-1,4-N-acetylglucosaminyltransferase; EC 2.4.1.155/alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase |
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