Document Detail


Determination of L-ascorbic acid in Lycopersicon fruits by capillary zone electrophoresis.
MedLine Citation:
PMID:  11554717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study shows an improved method for the determination of L-ascorbic acid (l-AA) in fruits of Lycopersicon by capillary zone electrophoresis (CZE). Two backgrounds electrolytes (BGEs) have been tested: (i) 400 mM borate at pH 8.0 and 1 x 10(-2)% hexadimethrine bromide, for the separation of Eulycopersicon subgenus species; and (ii) as in BGE(i) but supplemented with 20% (v/v) acetonitrile, for the separation of species of the Eriopersicon subgenus. The present procedures were compared with two routine methods-enzymatic assay and potentiometric titration with 2,6-dichlorophenol-indophenol. While these routine methods presented some difficulties in quantifying l-AA in several Lycopersicon fruits, CZE was successfully applied in all the analyzed samples. The proposed CZE protocols give lower detection limits (<0.4 microg ml(-1)); are cheaper, quicker, and highly reproducible; and can be applied to analyze large series of samples (ca. 50 samples per day) which is utmost importance, not only in screening trials for internal quality and tomato breeding programs, but also in systematic and routine characterization of Lycopersicon fruits.
Authors:
L Galiana-Balaguer; S Roselló; J M Herrero-Martínez; A Maquieira; F Nuez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  296     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-13     Completed Date:  2001-12-04     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-24     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Department of Biotechnology, Genetics, Polytechnic University of Valencia, Valencia, E-46022, Spain.
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / analysis*
Electrophoresis, Capillary / methods*
Fruit / chemistry
Lycopersicon esculentum / chemistry*
Potentiometry / methods
Chemical
Reg. No./Substance:
50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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