Document Detail

Determination of Indole Alkaloids and Highly Volatile Compounds in Rauvolfia verticillata by HPLC-UV and GC-MS.
MedLine Citation:
PMID:  23212134     Owner:  NLM     Status:  Publisher    
Rauvolfia verticillata (Lour.) Baill. (also called Luofumu in Chinese) is commonly used in traditional Chinese medicine for lowering blood pressure. In this study, a high-performance liquid chromatography assay using ultraviolet detection is described for the simultaneous measurement of the five bioactive indole alkaloids (sarpagine, yohimbine, ajmaline, ajmalicine and reserpine) in Rauvolfia. The detection of all five compounds was conducted at 280 nm. In quantitative analysis, the five compounds showed good regressions (R(2) > 0.9988) within the test ranges, and the recovery of the method was in the range of 90.4-101.4%. In addition, a simple gas chromatography mass method using a DB-1 silica capillary column (30 m × 0.25 mm i.d., 0.25 µm) is described for the identification of the highly volatile compounds in Rauvolfia. In qualitative analysis, more than 39 compounds were assayed and identified using the mass function and the National Institute of Standards and Technology database search system. The results demonstrated that the combination of quantitative and qualitative analyses offered an efficient way to evaluate the quality and consistency of Rauvolfia verticillata.
Bo Hong; Wenjing Li; Aihua Song; Chunjie Zhao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Journal of chromatographic science     Volume:  -     ISSN:  1945-239X     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Institute of Medicine, Qiqihar Medical University, Qiqihar 161042, China.
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