| Determination of a HIV protease inhibitor (DMP 450) in animal and human plasma by solid-phase extraction and high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 8738035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Extraction of DMP 450 from plasma was performed with C2 solid-phase extraction columns, using 0.1 M ammonium acetate in 90% methanol to elute DMP 450. The extraction recovery over the range of 10 to 10 000 ng/ml averaged 81.0, 96.2, 77.4, 95.2 and 68.0% from rat, dog, monkey, chimpanzee (25-10 000 ng/ml) and human plasma, respectively. HPLC analysis was carried out with a C18 column and a mobile phase of acetonitrile, methanol and 30 mM potassium phosphate (pH 3), the composition dependent on the type of plasma being analyzed, and monitored at a wavelength of 229 nm. Intra-day and inter-day coefficients of variation were less than 9.9 and 12.9%, respectively. Absolute differences were less than 11.5%. |
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Authors:
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M F Grubb; C A Robinson; S J White; C L Krauthauser; L Shum |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 678 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1997-01-28 Completed Date: 1997-01-28 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 303-8 Citation Subset: IM; X |
Affiliation:
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Drug Metabolism and Pharmacokinetics Section, DuPont Merck Pharmaceutical Company, Stine-Haskell Research Center, Newark, DE 19714, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetonitriles Animals Azepines / blood* Chromatography, High Pressure Liquid / methods*, statistics & numerical data Dogs HIV Protease Inhibitors / blood* Haplorhini Humans Hydrogen-Ion Concentration Methanol Pan troglodytes Phosphates Potassium Compounds Rats Sensitivity and Specificity Urea / analogs & derivatives*, blood |
| Chemical | |
Reg. No./Substance:
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0/Acetonitriles; 0/Azepines; 0/DMP 450; 0/HIV Protease Inhibitors; 0/Phosphates; 0/Potassium Compounds; 16068-46-5/potassium phosphate; 57-13-6/Urea; 67-56-1/Methanol; 75-05-8/acetonitrile |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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