Document Detail


Determination of a HIV protease inhibitor (DMP 450) in animal and human plasma by solid-phase extraction and high-performance liquid chromatography.
MedLine Citation:
PMID:  8738035     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extraction of DMP 450 from plasma was performed with C2 solid-phase extraction columns, using 0.1 M ammonium acetate in 90% methanol to elute DMP 450. The extraction recovery over the range of 10 to 10 000 ng/ml averaged 81.0, 96.2, 77.4, 95.2 and 68.0% from rat, dog, monkey, chimpanzee (25-10 000 ng/ml) and human plasma, respectively. HPLC analysis was carried out with a C18 column and a mobile phase of acetonitrile, methanol and 30 mM potassium phosphate (pH 3), the composition dependent on the type of plasma being analyzed, and monitored at a wavelength of 229 nm. Intra-day and inter-day coefficients of variation were less than 9.9 and 12.9%, respectively. Absolute differences were less than 11.5%.
Authors:
M F Grubb; C A Robinson; S J White; C L Krauthauser; L Shum
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical applications     Volume:  678     ISSN:  1572-6495     ISO Abbreviation:  J. Chromatogr. B, Biomed. Appl.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-01-28     Completed Date:  1997-01-28     Revised Date:  2007-10-16    
Medline Journal Info:
Nlm Unique ID:  9421796     Medline TA:  J Chromatogr B Biomed Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  303-8     Citation Subset:  IM; X    
Affiliation:
Drug Metabolism and Pharmacokinetics Section, DuPont Merck Pharmaceutical Company, Stine-Haskell Research Center, Newark, DE 19714, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetonitriles
Animals
Azepines / blood*
Chromatography, High Pressure Liquid / methods*,  statistics & numerical data
Dogs
HIV Protease Inhibitors / blood*
Haplorhini
Humans
Hydrogen-Ion Concentration
Methanol
Pan troglodytes
Phosphates
Potassium Compounds
Rats
Sensitivity and Specificity
Urea / analogs & derivatives*,  blood
Chemical
Reg. No./Substance:
0/Acetonitriles; 0/Azepines; 0/DMP 450; 0/HIV Protease Inhibitors; 0/Phosphates; 0/Potassium Compounds; 16068-46-5/potassium phosphate; 57-13-6/Urea; 67-56-1/Methanol; 75-05-8/acetonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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