Document Detail


Determination of fatty acids in human sweat during fasting using GC/MS.
MedLine Citation:
PMID:  20702949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fatty acids (FAs) are biological molecules that are used as major metabolic fuels, and are concerned in important metabolic processes. We have performed a non-invasive and technically rapid and simple method for collecting sweat from humans, followed by GC/MS determination. The sweat was collected from each volunteer (the middle finger) by spraying 70% ethanol aqueous solution (no harmful solvent) into a 1.5-cm(3) plastic vial. Analysis of FAs in sweat showed that the sweat solution contains lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (C18:0). Here, it is demonstrated that FA concentrations for 4 young subjects correlated positively with percent of body fat (r = 0.78) and that the total FA levels for them increased progressively with increasing fasting time when a subject fasted throughout the experiment.
Authors:
Yoko Nunome; Takao Tsuda; Kuniyuki Kitagawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  26     ISSN:  1348-2246     ISO Abbreviation:  Anal Sci     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-12     Completed Date:  2010-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  917-9     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan. nunome.yoko@b.mbox.nagoya-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue
Adult
Fasting
Fatty Acids / analysis*,  secretion
Female
Gas Chromatography-Mass Spectrometry / methods*
Humans
Male
Sweat / secretion*
Time Factors
Chemical
Reg. No./Substance:
0/Fatty Acids

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