| Determination of Domperidone in Human Plasma using Liquid Chromatography Coupled to Tandem Mass Spectrometry and its Pharmacokinetic Study. | |
| | |
MedLine Citation:
|
PMID: 22297684 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A sensitive and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of domperidone (CAS number: 57808-66-9) in human plasma using paracetamol (CAS number: 103-90-2) as an internal standard (IS). Domperidone and paracetamol in plasma were extracted with ethyl acetate, separated on a C18 reversed phase column, eluted with mobile phase of acetonitrile-glacial acetic acid (0.3%) (40:60, v/v), ionized by positive ion pneumatically assisted electrospray and detected in the multi-reaction monitoring mode using precursor→product ions of m/z 426.2→175.1 for domperidone and 152→110 for the IS, respectively. The calibration curve was linear (r2≥0.99, n=5) over the concentration range of 0.2-80 ng/mL and with lower limit of detection and quantitation of 0.05 and 0.2 ng/mL. The specificity, matrix effect, recovery, sensitivity, linearity, accuracy, precision, and stabilities were validated for domperidone in human plasma. In conclusion, the validation results showed that this method was sensitive, economical and less toxic and it can successfully fulfill the requirement of clinical pharmacokinetic study of domperidone oral preparation in Chinese healthy volunteers. |
| | |
Authors:
|
D Zhang; K Chen; Y Teng; J Zhang; S Liu; C Wei; B Wang; X Liu; G Yuan; R Zhang; R Guo |
Related Documents
:
|
22414024 - Consensus structure elucidation combining gc/ei-ms, structure generation and calculated... 7704214 - Simultaneous determination of dihydrocodeine and dihydromorphine in serum by gas chroma... 6233294 - Studies on steroids. cc. determination of 17-ketosteroid sulphates in serum by high-per... 22322824 - Measuring asymmetric dimethylarginine (adma) in ckd: a comparison between enzyme-linked... 20229384 - Long-term continuous sampling of ¹²co₂, ¹³co₂ and ¹²c¹⁸o¹⁶o in ambient ... 16599834 - Multicenter evaluation of the interference of hemoglobin, bilirubin and lipids on synch... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-1 |
Journal Detail:
|
Title: Arzneimittel-Forschung Volume: - ISSN: 0004-4172 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372660 Medline TA: Arzneimittelforschung Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© Georg Thieme Verlag KG Stuttgart · New York. |
Affiliation:
|
Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, P. R. China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Blood glutamate scavengers prolong the survival of rats and mice with brain-implanted gliomas.
Next Document: Engineering the work function of armchair graphene nanoribbons using strain and functional species: ...