| Determination of deoxynivalenol in infant cereal by immunoaffinity column cleanup and high-pressure liquid chromatography-UV detection. | |
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MedLine Citation:
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PMID: 20537262 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of deoxynivalenol (DON) in cereal-based baby food, a primary source of the first solid food for infants, was studied in order to develop a method to detect its presence at low concentrations. DON, produced primarily by Fusarium graminearum, is commonly isolated from grains and feed around the world and affects both animal and human health, producing diarrhea, vomiting, gastrointestinal inflammation, and immunomodulation. An aqueous extract of infant cereal was cleaned by means of an immunoaffinity chromatography column. After the eluate was evaporated and redissolved, DON was determined by high-pressure liquid chromatography-UV. The level of quantification for DON was 10 ppb for three types of infant cereal (mixed, barley, and oatmeal); the level of detection was 5 ppb. The protocol we have developed can measure DON between 10 to 500 ppb. An advisory level of 1 ppm for wheat products has been established by the U.S. Food and Drug Administration; however, the European Communities (EC) regulations have been set at 200 ppb for cereal-based foods for infants. Only 1 of 52 samples of barley-, mixed-, or oat-based infant cereal purchased in 2008 and 2009 in the United States exceeded the European standard. |
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Authors:
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Mary Ann Dombrink-Kurtzman; Stephen M Poling; David F Kendra |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of food protection Volume: 73 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-11 Completed Date: 2010-07-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 1073-6 Citation Subset: IM |
Affiliation:
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Mycotoxin Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604-3902, USA. Mary.Ann.DombrinkKurtman@ars.usda.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cereals
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chemistry*,
microbiology Chromatography, High Pressure Liquid Consumer Product Safety Food Contamination / analysis* Food Microbiology Humans Infant Infant Food / analysis* Risk Assessment Trichothecenes / analysis, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Trichothecenes; 51481-10-8/deoxynivalenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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