Document Detail


Determination of D,L-serine in midbrain of Parkinson's disease mouse by capillary electrophoresis with in-column light-emitting diode induced fluorescence detection.
MedLine Citation:
PMID:  19156646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A capillary electrophoresis method with in-column light-emitting diode induced fluorescence detection is described for simultaneous determination of D,L-serine in the midbrain of a Parkinson's disease mouse. D,L-Serine was derivatized with fluorescein isothiocyanate, and chiral separation and determination of D,L-serine derivatives were performed on a laboratory-built capillary electrophoresis system with in-column light-emitting diode induced fluorescence detector using gamma-cyclodextrin as chiral selector. Using this method, the levels of D- and L-serine in the midbrains of Parkinson's disease mice were determined. When compared to controls, the levels of D- and L-serine showed significant differences. The result suggested that the biosynthesis and the transportation of endogenous D,L-serine may participate in Parkinson's disease pathogenesis.
Authors:
Shuting Li; Qinglei Yu; Xin Lu; Shulin Zhao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  32     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  282-7     Citation Subset:  IM    
Affiliation:
College of Chemistry and Chemical Engineering, Guangxi, Normal University, Guilin, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry
Electrolytes
Electrophoresis, Capillary / methods*
Fluorescein-5-isothiocyanate / analysis*,  chemistry
Lasers, Semiconductor*
Male
Mesencephalon / chemistry*,  metabolism*
Mice
Mice, Inbred C57BL
Parkinson Disease / metabolism*
Serine / analysis*,  chemistry,  metabolism
Stereoisomerism
Chemical
Reg. No./Substance:
0/Electrolytes; 3326-32-7/Fluorescein-5-isothiocyanate; 56-45-1/Serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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