Document Detail

Determination of Cry9C protein in processed foods made with StarLink corn.
MedLine Citation:
PMID:  12374406     Owner:  NLM     Status:  MEDLINE    
StarLink (Aventis CropScience US) hybrid corn has been genetically modified to contain a pesticidal protein, Cry9C, which makes it more resistant than traditional varieties to certain types of corn insect pests. Unlike other varieties of genetically engineered corn, the U.S. Environmental Protection Agency authorized the use of StarLink corn for animal feed and industrial use only, not for human consumption. However, some Cry9C-containing corn was mistakenly or inadvertently comingled with yellow corn intended for human food use. Because corn containing the Cry9C construct was not approved for human use, the U.S. Food and Drug Administration considers food containing it to be adulterated. Consequently, this regulatory violation resulted in hundreds of recalls of corn-based products, such as taco shells, containing cry9C DNA. Detecting the novel protein in StarLink corn is an emerging issue; therefore, there is no standardized or established analytical method for detecting Cry9C protein in processed foods. We developed a procedure for quantitation of Cry9C protein, with validation data, in processed food matrixes with a limit of quantitation at 1.7 ng/g (ppb), using a commercial polyclonal antibody-based Cry9C kit that was intended for corn grain samples. Intra- and interassay coefficients of variation were 2.8 and 11.8%, respectively. Mean recoveries were 73 and 85% at 2 and 5 ng/g Cry9C fortifications, respectively, for 19 control non-StarLink corn-based matrixes. Our data demonstrate only 0-0.5% of Cry9C protein survived the processing of tortilla chips and soft tortillas made from 100% StarLink corn, resulting in levels from below the detection limit to 45 ppb.
Carmen Diaz; Cecilia Fernandez; Regina McDonald; Jupiter M Yeung
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  85     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2002 Sep-Oct
Date Detail:
Created Date:  2002-10-10     Completed Date:  2003-04-01     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1070-6     Citation Subset:  IM    
National Food Processors Association, Washington, DC 20005, USA.
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MeSH Terms
Bacterial Proteins / analysis*
Bacterial Toxins*
Endotoxins / analysis*
Enzyme-Linked Immunosorbent Assay
Food Analysis
Hemolysin Proteins
Indicators and Reagents
Reproducibility of Results
Zea mays / chemistry*,  genetics*
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Endotoxins; 0/Hemolysin Proteins; 0/Indicators and Reagents; 0/insecticidal crystal protein, Bacillus Thuringiensis

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