| Determination of carbohydrate-binding preferences of human galectins with carbohydrate microarrays. | |
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MedLine Citation:
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PMID: 20572248 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Galectins are a class of carbohydrate-binding proteins named for their galactose-binding preference and are involved in a host of processes ranging from homeostasis of organisms to immune responses. As a first step towards correlating the carbohydrate-binding preferences of the different galectins with their biological functions, we determined carbohydrate recognition fine-specificities of galectins with the aid of carbohydrate microarrays. A focused set of oligosaccharides considered relevant to galectins was prepared by chemical synthesis. Structure-activity relationships for galectin-sugar interactions were determined, and these helped in the establishment of redundant and specific galectin actions by comparison of binding preferences. Distinct glycosylations on the basic lactosyl motifs proved to be key to galectin binding regulation--and therefore galectin action--as either high-affinity ligands are produced or binding is blocked. High-affinity ligands such as the blood group antigens that presumably mediate particular functions were identified. |
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Authors:
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Tim Horlacher; Matthias A Oberli; Daniel B Werz; Lenz Kröck; Simone Bufali; Rashmi Mishra; Jens Sobek; Kai Simons; Mitsuomi Hirashima; Toshiro Niki; Peter H Seeberger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chembiochem : a European journal of chemical biology Volume: 11 ISSN: 1439-7633 ISO Abbreviation: Chembiochem Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: Germany |
Other Details:
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Languages: eng Pagination: 1563-73 Citation Subset: IM |
Affiliation:
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Federal Institute of Technology (ETH) Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blood Group Antigens
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metabolism Carbohydrates / chemistry* Galectins / metabolism* Humans Microarray Analysis* Oligosaccharides / chemical synthesis, metabolism* Protein Binding Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Blood Group Antigens; 0/Carbohydrates; 0/Galectins; 0/Oligosaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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