Document Detail


Determination of ABO genotypes by real-time PCR using allele-specific primers.
MedLine Citation:
PMID:  22177907     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2h for accurate ABO genotyping using 2.0ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.
Authors:
Tomonori Muro; Junko Fujihara; Shinji Imamura; Hiroaki Nakamura; Kaori Kimura-Kataoka; Tomoko Toga; Reiko Iida; Toshihiro Yasuda; Haruo Takeshita
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-16
Journal Detail:
Title:  Legal medicine (Tokyo, Japan)     Volume:  -     ISSN:  1873-4162     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889186     Medline TA:  Leg Med (Tokyo)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan; Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue 690-8510, Japan.
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