Document Detail


Determination of ABO genotypes by real-time PCR using allele-specific primers.
MedLine Citation:
PMID:  22177907     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2h for accurate ABO genotyping using 2.0ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.
Authors:
Tomonori Muro; Junko Fujihara; Shinji Imamura; Hiroaki Nakamura; Kaori Kimura-Kataoka; Tomoko Toga; Reiko Iida; Toshihiro Yasuda; Haruo Takeshita
Related Documents :
21801397 - Anthrax phylogenetic structure in northern italy.
22058207 - Acquired mutations in asxl1 in acute myeloid leukemia: prevalence and prognostic value.
22170597 - Polymorphism of the prion protein gene (prnp) in polish cattle affected by classical bo...
22244917 - Common variation in genes related to immune response and risk of childhood leukemia.
19917047 - A shared 336 kb haplotype associated with the belt pattern in three divergent cattle br...
19702537 - Polymorphisms in methotrexate pathways: what is clinically relevant, what is not, and w...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-16
Journal Detail:
Title:  Legal medicine (Tokyo, Japan)     Volume:  -     ISSN:  1873-4162     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889186     Medline TA:  Leg Med (Tokyo)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan; Criminal Investigation Laboratory, Shimane Prefectural Police Headquarters, Matsue 690-8510, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Development of multiplex assay with 16 SNPs on X chromosome for degraded samples.
Next Document:  Coronary Flow in Neonates with Impaired Intrauterine Growth.