| Determination of 9-cis beta-carotene and zeta-carotene in biological samples. | |
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MedLine Citation:
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PMID: 18280136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Concentrations of 9-cis beta-carotene (9-cis betaC) and zeta-carotene (zetaC) in biological samples may provide crucial information on the biological activities of these carotenoids. However, in high-performance liquid chromatography (HPLC) these carotenoids are often co-eluted. Therefore, there is an urgent need to develop a method for 9-cis betaC and zetaC quantitation. Both 9-cis betaC and zetaC have peak absorbance at 400 and 450 nm, respectively, whereas only 9-cis betaC has peak absorbance at 475 nm. We developed a HPLC method to quantitate 9-cis betaC and zetaC by using peak absorbance ratios. The 9-cis betaC/zetaC peak area was monitored at 475, 450 and 400 nm. The 9-cis betaC was quantified by using absorbance value at 475 nm; zetaC was then calculated from the 9-cis betaC/zetaC peak at 400 nm by subtracting 9-cis betaC contribution at 400 nm using the 400-nm/475-nm peak absorbance ratio of 9-cis betaC (0.39). This method was applied to determine 9-cis betaC and zetaC concentrations in serum and breast milk samples (n=12) from American lactating women and serum and breast adipose tissue samples (n=16) from Korean women with either benign or malignant breast tumors. 9-cis betaC concentrations in serum and breast milk of American women, and serum and adipose tissue of Korean women were 7.1+/-0.8 and 1.1+/-0.2 nM, and 15.6+/-1.1 nM and 0.2+/-0.1 nmol/g, respectively. zetaC concentrations in the above samples were 54.2+/-7.2 and 8.3+/-1.8 nM, and 49.0+/-3.9 nM and 0.3+/-0.1 nmol/g, respectively. |
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Authors:
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Jian Qin; Kyung-Jin Yeum; Elizabeth J Johnson; Norman I Krinsky; Robert M Russell; Guangwen Tang |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-02-14 |
Journal Detail:
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Title: The Journal of nutritional biochemistry Volume: 19 ISSN: 0955-2863 ISO Abbreviation: J. Nutr. Biochem. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-11 Completed Date: 2008-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010081 Medline TA: J Nutr Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 612-8 Citation Subset: IM |
Affiliation:
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Jean Mayer USDA-Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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chemistry Adolescent Adult Breast / chemistry Female Humans Milk, Human / chemistry Reproducibility of Results beta Carotene / analysis*, isolation & purification zeta Carotene / analysis*, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/zeta Carotene; 7235-40-7/beta Carotene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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