| Determination of the 8-methyl ether of xanthurenic acid in human serum by high-performance liquid chromatography with fluorescence detection. | |
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MedLine Citation:
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PMID: 8998563 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a simple and sensitive assay for the 8-methyl ether of xanthurenic acid in serum by high-performance liquid chromatography with fluorescence detection (excitation at 340 nm, emission at 450 nm). The compound under study in serum samples was extracted with Sep-Pak C18 cartridge and the extract was applied to an octadecylsilane-bonded column (Nucleosil 5C18, 150 x 4 mm I.D.). The mobile phase used was a mixture of 0.05 M sodium acetate buffer (pH 6.0), containing 5 mM sodium 1-octanesulfonate and 0.1 mM Na2EDTA, and acetonitrile (93:7, v/v). The 8-methyl ether of xanthurenic acid from serum samples was sufficiently separated to be clearly distinguishable. The quantification limit was 2 x 10(-14) mol, which was sensitive enough to detect 8-methyl ether of xanthurenic acid in serum from normal subjects. The method was applied to samples from patients with deficiency in tryptophan metabolism, xanthurenic acid/3-hydroxy kynurenineuria and showed a striking elevation in serum 8-methyl ether of xanthurenic acid. |
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Authors:
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J Imai; I Yoshida; K Murayama; Y Sakai; H Shimizu; T Sumi; T Iguchi; M Kawai; S Yamaguchi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical applications Volume: 679 ISSN: 1572-6495 ISO Abbreviation: J. Chromatogr. B, Biomed. Appl. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1997-01-21 Completed Date: 1997-01-21 Revised Date: 2007-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9421796 Medline TA: J Chromatogr B Biomed Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 204-7 Citation Subset: IM |
Affiliation:
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Gifu Prefectural Health and Environment Research Center, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Metabolism, Inborn Errors
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blood*,
diagnosis Chromatography, High Pressure Liquid / methods Ethers Humans Kynurenine / analogs & derivatives, blood, diagnostic use Sensitivity and Specificity Silicon Dioxide / chemistry Spectrometry, Fluorescence / methods Tryptophan / metabolism* Xanthurenates / blood*, diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Ethers; 0/Xanthurenates; 0/octadecylsilica; 343-65-7/Kynurenine; 484-78-6/3-hydroxykynurenine; 59-00-7/xanthurenic acid; 73-22-3/Tryptophan; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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