Document Detail


Determination of 4-aminobutyric acid, aspartate, glutamate and glutamine and their 13C stable-isotopic enrichment in brain tissue by gas chromatography-mass spectrometry.
MedLine Citation:
PMID:  3571396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A selected-ion monitoring method was developed for measuring 4-aminobutyric acid, aspartate, glutamate, and glutamine in brain tissue. Natural isotopes of these amino acids and their stable-isotopic enrichment following intravenous infusion of a precursor, [13C]glucose, were quantitated. Frozen mouse brain tissue was homogenized in cold 80% ethanol, and the supernatant, equivalent to 1 mg of wet weight brain tissue, was extracted using solid-phase bonded silica ion-exchange columns. Aspartate and glutamate (dicarboxylic acids) were isolated from strong anion-exchange columns, whereas 4-aminobutyric acid and glutamine (neutral amino acids) were isolated from strong-cation exchange columns. n-Butyl ester pentafluoropropionyl amide derivatives of these amino acids were analyzed by gas chromatography-mass spectrometry using a methane positive chemical ionization mode after gas chromatographic separation on a wide-bore, fused-silica capillary column. The method is applicable to determination of brain concentrations of these amino acids as well as their fluxes following administration of a stable-isotopic tracer.
Authors:
I M Kapetanovic; W D Yonekawa; H J Kupferberg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  414     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-06-18     Completed Date:  1987-06-18     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  265-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Animals
Aspartic Acid / analysis
Brain Chemistry*
Carbon Isotopes
Gas Chromatography-Mass Spectrometry
Glutamates / analysis
Glutamine / analysis
Male
Mice
gamma-Aminobutyric Acid / analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Glutamates; 0RH81L854J/Glutamine; 30KYC7MIAI/Aspartic Acid; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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