Document Detail


Determination of 2,5-hexanedione in urine by headspace solid-phase microextraction and gas chromatography.
MedLine Citation:
PMID:  19470226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
2,5-Hexanedione (2,5-HD) is the most important metabolite of n-hexane and methyl ethyl ketone in human urine. Urinary 2,5-HD is used as a biomarker for biological monitoring of workers exposed to n-hexane. A simple method using headspace solid-phase microextraction (HS-SPME) and gas chromatography (GC) equipped with a flame-ionization detector (FID) was developed. The parameters that affect the HS-SPME-GC-FID process were optimized (i.e., fiber coating, sample volume, adsorption and heating time, salt addition, and extraction temperature). The assay presented linearity in the range of 0.075 to 20.0 mg/L, precision (coefficient of variation < 7.0%), and detection limit of 0.025 mg/L for 2,5-HD in urine. The method was successfully applied to the analysis of 2,5-HD in urine samples from eight workers occupationally exposed to n-hexane in shoemaker's glue.
Authors:
Antônio Felipe F Oliveira; Patrícia P Maia; Maria José N Paiva; Maria Elisa P B Siqueira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  33     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-27     Completed Date:  2009-07-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  223-8     Citation Subset:  IM    
Affiliation:
Laboratory of Toxicological Analysis, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adhesives / metabolism*
Biological Markers / urine
Biotransformation
Calibration
Chromatography, Gas* / standards
Environmental Monitoring / methods*,  standards
Flame Ionization
Hexanes / metabolism*
Hexanones / urine*
Humans
Occupational Exposure*
Reproducibility of Results
Shoes
Solid Phase Microextraction* / standards
Chemical
Reg. No./Substance:
0/Adhesives; 0/Biological Markers; 0/Hexanes; 0/Hexanones; 110-13-4/2,5-hexanedione; 110-54-3/n-hexane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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