| Determination of 25-OH-PPD in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies. | |
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MedLine Citation:
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PMID: 17825630 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the investigation of the pharmacokinetics of 20(R)-dammarane-3beta,12beta,20,25-tetrol (25-OH-PPD) in rat. Ginsenoside Rh(2) was employed as an internal standard. The plasma samples were pretreated by liquid-liquid extraction and analyzed using LC/MS/MS with an electrospray ionization interface. The mobile phase consisted of methanol-acetonitrile-10 mmol/l aqueous ammonium acetate (42.5:42.5:15, v:v:v), which was pumped at 0.4 ml/min. The analytical column (50 mm x 2.1 mm i.d.) was packed with Venusil XBP C8 material (3.5 microm). The standard curve was linear from 10 to 3000 ng/ml. The assay was specific, accurate (accuracy between -1.19 and 2.57% for all quality control samples), precise and reproducible (within- and between-day precisions measured as relative standard deviation were <5% and <7%, respectively). 25-OH-PPD in rat plasma was stable over three freeze-thaw cycles and at ambient temperatures for 6h. The method had a lower limit of quantitation of 10 ng/ml, which offered a satisfactory sensitivity for the determination of (25-OH-PPD) in plasma. This quantitation method was successfully applied to pharmacokinetic studies of 25-OH-PPD after both an oral and an intravenous administration to rats and the absolute bioavailability is 64.8+/-14.3%. |
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Authors:
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Xiangrong Zhang; Dan Zhang; Jinghua Xu; Jingkai Gu; Yuqing Zhao |
Publication Detail:
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Type: Journal Article Date: 2007-08-23 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 858 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-09 Completed Date: 2007-12-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 65-70 Citation Subset: IM |
Affiliation:
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Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid / methods* Ginsenosides / blood*, chemistry, pharmacokinetics* Molecular Structure Rats Rats, Wistar Reproducibility of Results Tandem Mass Spectrometry / methods* |
| Chemical | |
Reg. No./Substance:
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0/Ginsenosides; 78214-33-2/ginsenoside Rh2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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