Document Detail


Determination of 17alpha-hydroxypregnenolone sulfate and its application in diagnostics.
MedLine Citation:
PMID:  17298837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New combined radioimmunoassay for determination of 17-hydroxypregnenolone sulfate (17-PregS) involving the hydrolysis of analyte by methanolysis was developed. 17-PregS, in addition to being secreted by the adrenals, is also formed by peripheral sulfoconjugation of 17-hydroxypregnenolone (17-Preg) or directly by hydroxylation of pregnenolone sulfate with 17alpha-hydroxylase/C17-20lyase. The measurement of 17-PregS can be used as a tool for detection of enzymatic deficiency particularly in pregnancy and for detection of congenital adrenal hyperplasia or gonadal dysfunction. The serum levels of 17-PregS, 17-Preg, dehydroepiandrosterone, dehydroepiandrosterone sulfate, pregnenolone and pregnenolone sulfate were measured in different age groups of human and in pregnant women respecting the age of gestation. The levels of 17-PregS are approximately three times higher than the levels of free 17-Preg in all subject groups. The levels of 17-PregS during pregnancy reached the local minimum in the 3rd month of gestation. The ratio of 17-PregS to free 17-Preg showed increasing profile during pregnancy with a maximum in the 8th month of gestation. These findings indicate that, the conversion of pregnenolone sulfate to 17-PregS is the major metabolic pathway for biosynthesis of 17-PregS.
Authors:
Helena Vceláková; Martin Hill; Oldrich Lapcík; Antonín Parízek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-12
Journal Detail:
Title:  Steroids     Volume:  72     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  323-7     Citation Subset:  IM    
Affiliation:
Institute of Endocrinology, Národní 8, 11694 Prague 1, Czech Republic. hhavlikova@endo.cz
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MeSH Terms
Descriptor/Qualifier:
17-alpha-Hydroxypregnenolone / analogs & derivatives*,  blood,  metabolism
Adolescent
Adult
Age Factors
Child
Child, Preschool
Female
Gestational Age
Humans
Male
Menstrual Cycle
Middle Aged
Pregnancy
Radioimmunoassay / methods*
Reference Values
Sex Factors
Chemical
Reg. No./Substance:
28901-70-4/17-hydroxypregnenolone sulfate; 387-79-1/17-alpha-Hydroxypregnenolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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