| Determination of 17alpha-hydroxypregnenolone sulfate and its application in diagnostics. | |
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MedLine Citation:
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PMID: 17298837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New combined radioimmunoassay for determination of 17-hydroxypregnenolone sulfate (17-PregS) involving the hydrolysis of analyte by methanolysis was developed. 17-PregS, in addition to being secreted by the adrenals, is also formed by peripheral sulfoconjugation of 17-hydroxypregnenolone (17-Preg) or directly by hydroxylation of pregnenolone sulfate with 17alpha-hydroxylase/C17-20lyase. The measurement of 17-PregS can be used as a tool for detection of enzymatic deficiency particularly in pregnancy and for detection of congenital adrenal hyperplasia or gonadal dysfunction. The serum levels of 17-PregS, 17-Preg, dehydroepiandrosterone, dehydroepiandrosterone sulfate, pregnenolone and pregnenolone sulfate were measured in different age groups of human and in pregnant women respecting the age of gestation. The levels of 17-PregS are approximately three times higher than the levels of free 17-Preg in all subject groups. The levels of 17-PregS during pregnancy reached the local minimum in the 3rd month of gestation. The ratio of 17-PregS to free 17-Preg showed increasing profile during pregnancy with a maximum in the 8th month of gestation. These findings indicate that, the conversion of pregnenolone sulfate to 17-PregS is the major metabolic pathway for biosynthesis of 17-PregS. |
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Authors:
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Helena Vceláková; Martin Hill; Oldrich Lapcík; Antonín Parízek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-12 |
Journal Detail:
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Title: Steroids Volume: 72 ISSN: 0039-128X ISO Abbreviation: Steroids Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-27 Completed Date: 2007-07-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404536 Medline TA: Steroids Country: United States |
Other Details:
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Languages: eng Pagination: 323-7 Citation Subset: IM |
Affiliation:
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Institute of Endocrinology, Národní 8, 11694 Prague 1, Czech Republic. hhavlikova@endo.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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17-alpha-Hydroxypregnenolone
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analogs & derivatives*,
blood,
metabolism Adolescent Adult Age Factors Child Child, Preschool Female Gestational Age Humans Male Menstrual Cycle Middle Aged Pregnancy Radioimmunoassay / methods* Reference Values Sex Factors |
| Chemical | |
Reg. No./Substance:
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28901-70-4/17-hydroxypregnenolone sulfate; 387-79-1/17-alpha-Hydroxypregnenolone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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