| Determination of 17-oxosteroids in serum and urine by fluorescence high-performance liquid chromatography using dansyl hydrazine as a pre-labeling reagent. | |
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MedLine Citation:
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PMID: 7320136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fluorescence high-performance liquid chromatographic method is described for the determination of 17-oxosteroids in biological fluids. 17-Oxosteroids in urine samples are extracted with dichloromethane after enzymatic hydrolysis (beta-glucuronidase-sulfatase), and dehydroepiandrosterone sulfate in serum samples is solvolysed with sulfuric acid in ethyl acetate. 17-Oxosteroids are labeled with dansyl hydrazine in trichloroacetic acid-benzene solution, and then chromatographed on the microparticulate silica gel column using dichloromethane-ethanol-water (400 : 1 : 2) as the mobile phase. The eluate is monitored by a fluorophotometer at 365 nm (excitation) and 505 nm (emission). Linearities of the fluorescence intensities (peak heights) with the amounts of various 17-oxosteroids were obtained between 60 and 1000 pg. The assay proved satisfactory with respect to sensitivity, precision and accuracy. The results obtained by a radioimmunoassay and this method were in good agreement (r = 0.964, n = 81) for serum dehydroepiandrosterone sulfate. This method is also use for the simultaneous determination of individual 17-oxosteroids in serum and urine. |
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Authors:
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T Kawasaki; M Maeda; A Tsuji |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 226 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1981 Nov |
Date Detail:
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Created Date: 1982-03-22 Completed Date: 1982-03-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-12 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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17-Ketosteroids
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blood*,
urine Chromatography, High Pressure Liquid / methods Dansyl Compounds* Female Fluorescence Humans Hydrazines Male Reference Values |
| Chemical | |
Reg. No./Substance:
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0/17-Ketosteroids; 0/Dansyl Compounds; 0/Hydrazines; 33008-06-9/dansyl hydrazine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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