Document Detail

Determination of 13C isotopic enrichment of valine and threonine by GC-C-IRMS after formation of the N(O,S)-ethoxycarbonyl ethyl ester derivatives of the amino acids.
MedLine Citation:
PMID:  17468859     Owner:  NLM     Status:  MEDLINE    
We describe a new method of assessing, in a single run, (13)C isotopic enrichment of both Val and Thr by gas chromatography-combustion-isotope-ratio mass spectrometry (GC-C-IRMS). This method characterised by a rapid one-step derivatisation procedure performed at room temperature to form the N(O,S)-ethoxycarbonyl ethyl ester derivatives, and a polar column for GC. The suitability of this method for Val and Thr in in-vivo samples (mucosal hydrolysate) was demonstrated by studying protein metabolism with two tracers ((13)C-valine or (13)C-threonine). The intra-day and inter-day repeatability were both assessed either with standards or with in-vivo samples at natural abundance and at low (13)C isotopic enrichment. For inter-day repeatability CVs were between 0.8 and 1.5% at natural abundance and lower than 5.5% at 0.112 and 0.190 atom% enrichment for Val and Thr, respectively. Overall isotopic precision was studied for eleven standard amino acid derivatives (those of Val, Ala, Leu, Iso, Gly, Pro, Asp, Thr, Ser, Met, and Phe) and was assessed at 0.32 per thousand. The (13)C isotopic measurement was then extended to the other amino acids (Ala, Val, Leu, Iso, Gly, Pro, Thr, and Phe) at natural abundance for in-vivo samples. The isotopic precision was better than 0.002 atom% per amino acid (for n = 4 rats). This analytical method was finally applied to an animal study to measure Thr utilization in protein synthesis.
Jean-Philippe Godin; Magali Faure; Denis Breuille; Gérard Hopfgartner; Laurent-Bernard Fay
Related Documents :
18464529 - Comparison of preparation methods for the evaluation of nerve axons.
25188779 - Oxidation mechanism of formic acid on the bi-adatom modified pt(111) surface.
8136919 - Comparison of high-performance liquid chromatography with radioimmunoassay for the dete...
21331099 - Histone deacetylase inhibitor activity in royal jelly might facilitate caste switching ...
17889819 - A high-throughput method for the quantitative analysis of indole-3-acetic acid and othe...
17468859 - Determination of 13c isotopic enrichment of valine and threonine by gc-c-irms after for...
25392109 - Omega-3 fatty acids: a novel resort against gastrointestinal injury.
12640039 - Parp-3 localizes preferentially to the daughter centriole and interferes with the g1/s ...
7221659 - Acute obstructive uric acid nephropathy after treatment of neoplastic adenopathy.
Publication Detail:
Type:  Journal Article     Date:  2007-04-28
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  388     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-09-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  909-18     Citation Subset:  IM    
Department of Bioanalytical Science, Nestlé Research Center, Nestec Ltd, Vers chez les Blanc, Lausanne 26, Switzerland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / chemistry*
Carbon Isotopes / chemistry*
Esters / chemistry*
Ethyl Ethers / chemistry*
Gas Chromatography-Mass Spectrometry / methods*
Intestinal Mucosa / chemistry,  metabolism
Protein Biosynthesis
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Threonine / analysis*
Time Factors
Valine / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Carbon Isotopes; 0/Esters; 0/Ethyl Ethers; 7004-03-7/Valine; 72-19-5/Threonine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of s...
Next Document:  Bioanalytical procedures for determination of drugs of abuse in blood.