| Determination of 10-hydroxystearic, 10-ketostearic, 8-hydroxypalmitic, and 8-ketopalmitic acids in milk fat by solid-phase extraction plus gas chromatography-mass spectrometry. | |
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MedLine Citation:
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PMID: 21943732 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fatty acids (FA) bearing oxygenated functions and present in esterified form in triacylglycerols are widespread in nature but very little is known about their occurrence in dairy products. A method based on gas chromatography (with flame ionization detector and mass spectrometry detectors), including the previous isolation of polar FA methyl esters by solid-phase extraction, was applied to quantify oxygenated FA in milk fat. Samples obtained from ewes and goats fed with a variety of oil sources were studied. Fatty acids identified were 8-ketopalmitic, 8-hydroxypalmitic, 10-ketostearic, and mainly 10-hydroxystearic acids. The highest levels of 10-ketostearic acid were obtained in milk from animals fed olive oil (up to 1.5%) and from those fed long-chain n-3 FA-enriched diets (0.5-1.0%). In all samples, 10-hydroxystearic acid, not reported so far in milk, was the second most abundant oxygenated FA (up to 0.8%). The high correlation obtained between contents of 10-ketostearic and 10-hydroxystearic acids would confirm the existence of a common pathway of formation in the rumen, whereas the presence of 8-ketopalmitate and 8-hydroxypalmitate could be putatively attributed to mechanisms of β-oxidation in the tissues. The influence of cis-9 C18:1 and trans-10 C18:1 as precursors of these compounds in milk and the metabolic pathways involved in their formation are discussed. |
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Authors:
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G Márquez-Ruiz; V Rodríguez-Pino; M A de la Fuente |
Publication Detail:
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Type: - |
Journal Detail:
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Title: Journal of dairy science Volume: 94 ISSN: 1525-3198 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: - |
Other Details:
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Languages: ENG Pagination: 4810-4819 Citation Subset: - |
Copyright Information:
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Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Instituto de Ciencia y Tecnología de Alimentos y Nutrición (CSIC), José Antonio Novais 10, 28040, Madrid, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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