Document Detail

Determinants of intestinal mucosal uptake of short- and medium-chain fatty acids and alcohols.
MedLine Citation:
PMID:  4715329     Owner:  NLM     Status:  MEDLINE    
Uptake rates across the jejunal brush border have been measured for water-soluble fatty acids and alcohols and analyzed to determine the relative roles of the unstirred water layer and the lipid cell membrane as determinants of the intestinal absorptive process. Initial studies involving measurement of time courses of electrical transients developed across the intestine exposed to poorly permeant solute molecules showed no anomalous discrimination of probe molecules of different size or charge. This finding suggests that the diffusion barrier in the intestine can be considered as an unstirred water layer. Next, uptake rates of fatty acid were found to be linear with respect to concentration of the test solute, demonstrated no competitive inhibition or contralateral stimulation, had low temperature dependency, and were insensitive to metabolic inhibition, indicating that uptake proceeds by passive diffusion. Passive permeability coefficients, *P, varied from 22 +/- 1.4 to 395 +/- 9.2 nmoles.min(-1).100 mg(-1).mm(-1) for the saturated fatty acids 2:0 through 12:0 and from 119 +/- 3.3 to 581 +/- 45.2 for the saturated alcohols 6:0 through 10:0. Vigorous stirring of the bulk buffer solution enhanced *P values in direct proportion to chain length while the presence of bile acid micelles depressed apparent permeability coefficients in proportion to fatty acid chain length. These results demonstrate that uptake of short-chain fatty acid monomers is rate limited by the lipid cell membrane but diffusion through the unstirred water layer becomes increasingly rate limiting as the chain length increases. It is also possible to conclude from these data that diffusion through the unstirred water layer becomes totally rate limiting for uptake of long-chain fatty acid monomers of physiological importance.
V L Sallee; J M Dietschy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  14     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1973 Jul 
Date Detail:
Created Date:  1973-09-04     Completed Date:  1973-09-04     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  475-84     Citation Subset:  IM    
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MeSH Terms
Biological Transport
Carbon Isotopes
Cell Membrane Permeability
Dextrans / metabolism
Fatty Acids, Nonesterified / metabolism*
Fatty Alcohols / metabolism*
Hydrogen-Ion Concentration
Intestinal Absorption*
Intestinal Mucosa / drug effects,  metabolism*
Jejunum / metabolism
Molecular Conformation
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids, Nonesterified; 0/Fatty Alcohols; 10028-17-8/Tritium; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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