| Determinants of the in vitro interaction of polyaspartic acid and aminoglycoside antibiotics. | |
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MedLine Citation:
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PMID: 1469645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The in vitro interaction of polyaspartic acid (PAA) with aminoglycosides was evaluated using double diffusion in agar, dialysis chambers and changes in the optical density of test solutions. The results document a reversible, presumably electrostatic interaction that is optimized at a pH of approximately 5.0, by the absence of proteins over 800 Da, and at a 20:1 or 10:1 molar ratio of aminoglycoside to PAA. The PAA-aminoglycoside complex lost antibacterial activity and the ability to inhibit pronase E enzymatic activity. These results allow generation of a hypothesis as to the mechanism whereby PAA prevents aminoglycoside experimental nephrotoxicity. |
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Authors:
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S J Kohlhepp; D N McGregor; S J Cohen; M E Kohlhepp; D N Gilbert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: 263 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1993-01-22 Completed Date: 1993-01-22 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1464-70 Citation Subset: IM |
Affiliation:
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Infectious Diseases Research Laboratory, Chiles Research Institute, Providence Medical Center, Portland, Oregon. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminoglycosides Anti-Bacterial Agents / pharmacology*, toxicity Blood Physiological Phenomena Drug Interactions Hydrogen-Ion Concentration Kidney / drug effects Peptides / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Aminoglycosides; 0/Anti-Bacterial Agents; 0/Peptides; 26063-13-8/polyaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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