Document Detail

Determinants of biliary secretion in isolated perfused skate liver.
MedLine Citation:
PMID:  7065254     Owner:  NLM     Status:  MEDLINE    
In the isolated perfused liver of the little skate, Raja erinacea, bile flow averaged 5.07 +/- 0.58 (mean +/- SE) microliters.h-1.g liver-1 in 21 experiments at a perfusion pressure of 5.0 cm Ringer compared to 3.79 +/- 0.32 in 38 experiments at 2.5 cm (P less than 0.05). [14C]inulin readily entered skate bile. Bile-to-plasma [14C]inulin ratios corrected for delay in transit time, averaged 0.46 +/- 0.07 at 1 h and rose to 0.74 +/- 0.06 by 4 h, although bile flow remained constant. In experiments in which [14C]inulin reached equilibrium between bile and plasma, the bile-to-plasma ratio conformed to the theoretical relationship between bile flow, solvent drag, and inert solute diffusion predicted at extremely low bile flows, but demonstrated that the skate biliary tree is more permeable to inulin than that of the rat. Electron microscopic studies demonstrated that ionic lanthanum could traverse the tight junctions. However, freeze-fracture studies of junction structure did not differ qualitatively from similar studies in the rat. Partial dependence of bile flow on perfusion pressure, high bile-to-plasma inulin ratios, and permeability of the canalicular tight junctions to ionic lanthanum all suggest that the paracellular pathway may be an important component of bile formation in the skate.
J S Reed; N D Smith; J L Boyer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  242     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-05-27     Completed Date:  1982-05-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G319-25     Citation Subset:  IM    
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MeSH Terms
Bile / drug effects,  secretion*
Bile Ducts / physiology*,  ultrastructure
Dinitrophenols / pharmacology
Freeze Fracturing
Liver / drug effects,  physiology*
Microscopy, Electron
Potassium Cyanide / pharmacology
Uncoupling Agents / pharmacology
Grant Support
Reg. No./Substance:
0/Dinitrophenols; 0/Uncoupling Agents; 151-50-8/Potassium Cyanide; 51-28-5/2,4-Dinitrophenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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