Document Detail

Detection of Wolbachia DNA in blood for diagnosing filaria-associated syndromes in cats.
MedLine Citation:
PMID:  22649020     Owner:  NLM     Status:  MEDLINE    
A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in the pathogenesis of Dirofilaria immitis infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to carry out a molecular survey in a convenience sample of cats living in an area where D. immitis is endemic and to evaluate the detection of bacterial DNA in blood as a surrogate assay for diagnosing filaria-associated syndromes in cats. COI and FtsZ loci were used as targets for D. immitis and Wolbachia PCR assays, respectively, and real-time TaqMan PCR assays were used only for Wolbachia. A convenience sample of 307 disease-affected or healthy cats examined at a University facility were PCR tested, and their medical records were investigated. Conventional nested PCR for Wolbachia amplified the endosymbionts of both D. immitis and D. repens, while real-time PCR was highly specific only for the former. Observed prevalences of 0.3 and 10.4% were found using conventional nested PCR assays for D. immitis and real-time PCR for Wolbachia, respectively. Similar prevalences were established using the Wolbachia nested PCR (98% concordance with real-time PCR). The group of Wolbachia-positive samples had a significantly higher proportion of subjects with respiratory signs (29.0% versus 9.7%; P = 0.002). The findings of this study indicate that a highly sensitive PCR assay can be used to detect the Wolbachia organism in the peripheral blood of cats with respiratory signs.
Maria Elena Turba; Elisa Zambon; Augusta Zannoni; Samanta Russo; Fabio Gentilini
Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2012-05-30
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-25     Completed Date:  2012-11-28     Revised Date:  2013-06-24    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2624-30     Citation Subset:  IM    
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy.
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MeSH Terms
Bacterial Proteins / genetics
Blood / microbiology*
Cat Diseases / diagnosis*
DNA, Bacterial / genetics,  isolation & purification*
Dirofilaria immitis / microbiology*
Filariasis / diagnosis,  veterinary*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Veterinary Medicine / methods
Wolbachia / genetics,  isolation & purification*
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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