Document Detail


Detection and widespread distribution of Chlamydia pneumoniae in the vascular system and its possible implications.
MedLine Citation:
PMID:  8655672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To attempt to detect Chlamydia pneumoniae DNA in atheromatous vascular tissue. METHODS: A modification of an existing polymerase chain reaction (PCR) assay and immunofluorescence staining with a monoclonal antibody directed against C pneumoniae were used to detect C pneumoniae. Specimens from 32 patients undergoing abdominal aortic aneurysm repair were examined. Vascular tissue, ostensibly normal, from six liver transplant donors was also examined for comparison. Altogether, 43 vessels from these 38 subjects (age range 36-85 years) were examined. RESULTS: C pneumoniae was detected in 11 (44%) of 25 aortas, five (55%) of nine iliac arteries, two (40%) of five femoral arteries, and one of two iliac veins. Immunofluorescence staining supported positive PCR results in three of 12 cases in which it was used. Overall, C pneumoniae was detected in the arteries of 14 (44%) of the patients undergoing vascular surgery and three (50%) of the donors. CONCLUSIONS: This study is the first in the UK in which C pneumoniae organisms have been found in atherosclerotic vessels and the tendency for the organisms to be present most often in such vessels exhibiting chronic inflammatory changes suggests that a search for them in various forms of arteritis may also be rewarding.
Authors:
G Ong; B J Thomas; A O Mansfield; B R Davidson; D Taylor-Robinson
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  49     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-07-31     Completed Date:  1996-07-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  102-6     Citation Subset:  AIM; IM    
Affiliation:
Department of Genitourinary Medicine, St Mary's Hospital Medical School, London.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Aorta / microbiology
Aortic Aneurysm / surgery
Arteriosclerosis / microbiology*
Base Sequence
Chlamydophila pneumoniae / isolation & purification*
Female
Femoral Artery / microbiology
Humans
Iliac Artery / microbiology
Iliac Vein / microbiology
Male
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction
Comments/Corrections

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