Document Detail


Detection of walnut residues in foods using an enzyme-linked immunosorbent assay.
MedLine Citation:
PMID:  19723237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tree nuts, including walnuts, can be responsible for allergic reactions. Food manufacturers have the responsibility to declare the presence of walnuts on packaged foods even when trace residues may be present from the use of shared equipment or the adventitious contamination of ingredients. The aim of this study was to develop a rapid, sensitive, and specific enzyme-linked immunosorbent assay (ELISA) method for the detection of walnut protein residues. Mixtures of raw and roasted English walnuts of several varieties were defatted, powdered, and used as separate antigens in sheep and New Zealand white rabbits. An ELISA was developed using the sheep antiroasted walnut serum as the capture reagent and rabbit antiroasted walnut serum as the detector reagent followed by addition of commercial goat anti-rabbit IgG antibody labeled with alkaline phosphatase and subsequent substrate addition. The performance of the ELISA was validated by testing known amounts of walnut (0 to 100 ppm) either spiked into or manufactured into milk chocolate, cookies, muffins, or ice cream. Recoveries of 1 to 100 ppm walnut-in-chocolate ranged from 71.6% to 119%+/- 7% to 16.5%. The walnut ELISA has a detection limit of 1 ppm (1 microg/g) walnut in several food matrices. Substantial cross-reactivity was observed with pecan while minimal cross-reactivity was noted for hazelnut, mustard, mace, and poppy seed among almost 100 foods and food ingredients tested. This walnut ELISA can be used to detect undeclared walnut residues in foods and ingredients and as a tool to validate the effectiveness of allergen control programs for walnuts.
Authors:
Lynn Niemann; Steve L Taylor; Susan L Hefle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food science     Volume:  74     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  T51-7     Citation Subset:  IM    
Affiliation:
Food Allergy Research & Resource Program, Dept. of Food Science and Technology, Univ. of Nebraska-Lincoln, Lincoln, NE 68583-0919, USA.
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis*
Animals
Antigens, Plant / analysis*
Cross Reactions
Enzyme-Linked Immunosorbent Assay*
Food Analysis / methods*
Food Contamination / prevention & control
Food Handling
Juglans / chemistry*,  immunology
Nut Hypersensitivity / prevention & control*
Nuts / chemistry*,  immunology
Sanitation
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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