| Detection of vitellogenin and zona radiata protein expressions in surface mucus of immature juvenile Atlantic salmon (Salmo salar) exposed to waterborne nonylphenol. | |
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MedLine Citation:
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PMID: 15871908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Induction of blood plasma vitellogenin (Vtg) and zona radiata proteins (Zr-proteins) in male and juvenile of oviparous vertebrates was proposed and shown to be sensitive biomarkers for exposure to estrogen mimic. The time- and dose-dependent expression of Vtg and Zr-proteins in nonylphenol (NP) exposed juvenile Atlantic salmon (Salmo salar) is reported. Fish were exposed continuously to waterborne nonylphenol at 5, 15 and 50 microg/L. Blood and surface mucus samples were collected after 3 and 7 days post-exposure. Nonylphenol-induced plasma and surface mucus levels of Vtg and Zr-protein were analysed using immunochemical methods (Western blot and enzyme-linked immunosorbent assay; ELISA). Both Vtg and Zr-protein levels in plasma and surface mucus showed similar and parallel nonylphenol-induced expression patterns after waterborne nonylphenol exposure and in a concentration- and time-dependent manner. Zr-proteins were significantly induced at the lowest concentration of nonylphenol after 3 and 7 days of exposure both in plasma and in surface mucus. We conclude that the detection of Vtg and Zr-proteins directly in the surface mucus of fish, and the correlation of these values with plasma protein biomarker values in xenoestrogen-treated fish represents a sensitive non-invasive system for the detection of these known endocrine disruptor biomarkers. The demonstration of detectable Vtg and Zr-protein levels from surface mucus is a potential biomarker for estrogenic compounds, and their presence should be considered as an improvement in the methods for detection of endocrine disrupting chemicals (EDCs) and related pollutants in the environment. |
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Authors:
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Valentina Meucci; Augustine Arukwe |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Aquatic toxicology (Amsterdam, Netherlands) Volume: 73 ISSN: 0166-445X ISO Abbreviation: Aquat. Toxicol. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-16 Completed Date: 2005-08-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8500246 Medline TA: Aquat Toxicol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Affiliation:
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Department of Veterinary Clinics, Section of Pharmacology and Toxicology, University of Pisa, V. le delle Piagge 2, 56124 Pisa, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dose-Response Relationship, Drug Egg Proteins / blood, metabolism* Environmental Monitoring / methods Enzyme-Linked Immunosorbent Assay Gene Expression Regulation / drug effects* Immunoblotting Immunohistochemistry Mucus / metabolism* Phenols / toxicity* Salmo salar / metabolism* Time Factors Vitellogenins / blood, metabolism* Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Egg Proteins; 0/Phenols; 0/Vitellogenins; 0/Water Pollutants, Chemical; 0/zona radiata protein, fish; 25154-52-3/nonylphenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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