Document Detail


Detection of very early antibody to native HIV antigens by HIV neutralization and live-cell immunofluorescence assays.
MedLine Citation:
PMID:  16513186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Very early detection of HIV infection could help decrease the spread of HIV, improve safety of the blood supply, and permit earlier treatment. Early detection was reported when native gp41 antigen was used to detect antibodies that occurred 2-6 weeks earlier than detection of antibodies to denatured antigens by the current EIA or WB tests or detection by the HIV RNA test. We hypothesized that early antibodies to native gp41/160 could be detected, not only by the reported live-cell immunofluorescence (IFA) but also by a neutralization test, since virions as well as HIV-infected cells contain native gp41/160. To test this hypothesis, we did an initial test of concept study to compare the neutralization test with other tests, using sera from 12 high-risk patients. The neutralization test reproducibly detected early antibodies (characterized) in the sera of 10 of 12 (83%) high-risk subjects. Importantly, the EIA and WB tests that use denatured antigens missed the early diagnosis in 12 of the 13 subjects (92%). The findings support the concept that native HIV antigens can detect polyclonal HIV neutralizing antibodies and live-cell IFA antibodies earlier than currently available tests that use denatured antigens.
Authors:
Jingzhi Pan; Jianmin Chen; Hung Lee; Gautam K Sahu; Joyce S Poast; Stephen Tyring; Miles Cloyd; Samuel Baron
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-01-26
Journal Detail:
Title:  Antiviral research     Volume:  70     ISSN:  0166-3542     ISO Abbreviation:  Antiviral Res.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-04-20     Completed Date:  2006-08-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8109699     Medline TA:  Antiviral Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  21-7     Citation Subset:  IM    
Affiliation:
University of Texas Medical Branch, Department of Microbiology & Immunology, 301 University Blvd., Galveston, TX 77555-1070, USA. jpan@sial.com
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MeSH Terms
Descriptor/Qualifier:
Antibody Affinity / immunology
Early Diagnosis
Epitopes
Fluorescent Antibody Technique
HIV Antibodies / blood*,  immunology
HIV Envelope Protein gp41 / immunology*
HIV Infections / blood,  immunology*
Humans
Immunoglobulin G / blood,  immunology
Neutralization Tests / methods*
Chemical
Reg. No./Substance:
0/Epitopes; 0/HIV Antibodies; 0/HIV Envelope Protein gp41; 0/Immunoglobulin G

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