| Detection of trisomy 12 and Rb-deletion in CD34+ cells of patients with B-cell chronic lymphocytic leukemia. | |
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MedLine Citation:
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PMID: 9192749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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B-cell chronic lymphocytic leukemia (B-CLL) is a slowly progressive disease characterized by the clonal expansion of CD5+/CD23+ B lymphocytes. The malignant transformation is assumed to occur at the level of mature B lymphocytes. We asked whether CD34+ progenitor cells are involved in the malignant process in B-CLL. Furthermore, we investigated the possibility of aberrant CD34 expression by the malignant B-cell clone. Bone marrow and peripheral blood samples from 75 patients with B-CLL were tested for the presence of trisomy 12 and deletion of the retinoblastoma gene (Rb) by fluorescence in situ hybridization. CD34+ subpopulations were isolated by fluorescence-activated cell sorting and analyzed for the presence of the informative genetic marker. Bone marrow and peripheral blood samples of 10 B-CLL patients were analyzed for coexpression of CD34/CD5/CD20. Trisomy 12 was detected in 15 of 75 (20%) and Rb-deletion was detected in 6 of 30 patients (20%). In 7 patients with trisomy 12, hematopoietic progenitor cells were sorted, with the sort purity being between 85% and 99.8%. The genetic marker was detected in the CD34+/CD38+ cells as well as in the CD34+/38- subsets in 3 patients. Progenitor cells were also sorted in 2 patients with Rb-deletion. In 1 patient, Rb-deletion was present in 10% of CD34+/38+ cells. In the other patient, Rb-deletion was neither detected in the CD34+/38+ nor in the CD34+/CD38- subsets. In all 10 patients investigated for coexpression of CD34/CD5/CD20, we could not find a subpopulation coexpressing these markers. We conclude that trisomy 12 and Rb-deletion are present in a considerable subset of patients with B-CLL. In part of these patients, the genetic marker was detected at the level of CD34+ stem cells. CD34 expression is not related to an aberrant phenotype of the malignant B-cell clone. These results suggest that the malignant transformation in B-CLL may involve early hematopoietic stem cells and place a note of caution on future strategies using autologous stem cell transplantation. |
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Authors:
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B Gahn; C Schäfer; J Neef; C Troff; M Feuring-Buske; W Hiddemann; B Wörmann |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Blood Volume: 89 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1997 Jun |
Date Detail:
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Created Date: 1997-07-10 Completed Date: 1997-07-10 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4275-81 Citation Subset: AIM; IM |
Affiliation:
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Department of Hematology and Oncology, University of Göttingen, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Antigens, CD34 / analysis* B-Lymphocytes / ultrastructure* Cell Differentiation Cell Transformation, Neoplastic / genetics Chromosomes, Human, Pair 12* / genetics Female Gene Deletion Genes, Retinoblastoma* Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells / ultrastructure* Humans In Situ Hybridization, Fluorescence Leukemia, Lymphocytic, Chronic, B-Cell / genetics*, pathology Male Middle Aged Neoplastic Stem Cells / ultrastructure* Trisomy* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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