Document Detail


Detection of a transforming gene product in cells transformed by Moloney murine sarcoma virus.
MedLine Citation:
PMID:  6288258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We identified, in cells transformed by Moloney murine sarcoma virus (M-MuSV clone 124), a protein encoded by the M-MuSV transforming gene, v-mos. An antiserum against a synthetic peptide corresponding to the C terminus of a protein predicted from the v-mos nucleotide sequence specifically recognizes a protein doublet of approximately 37,000 daltons from 35S-methionine-labeled M-MuSV 124-transformed producer cells. By peptide mapping, this protein is almost identical to the 37 kd in vitro translation product from the M-MuSV v-mos gene. Immunoprecipitates from 32P-labeled cells contain a single v-mos-specific phosphoprotein, which has at least six sites of phosphorylation containing phosphoserine. Pulse-chase experiments show that the lower band in the 35S-methionine-labeled doublet is the primary translation product, which is modified, probably by phosphorylation, to yield the upper band. A similar mos protein is immunoprecipitated from HT1-MuSV-transformed cells, but not from uninfected NIH/3T3 cells. These mos proteins are present at very low levels in transformed cell lines. Cells acutely infected with M-MuSV 124, however, transiently contain much higher levels of the mos protein. These high levels coincide with extensive cell mortality.
Authors:
J Papkoff; I M Verma; T Hunter
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  29     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1982 Jun 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  417-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Transformation, Viral*
Cells, Cultured
Fibroblasts / metabolism
Mice
Moloney murine leukemia virus / metabolism*
Phosphoproteins / biosynthesis
Phosphorylation
Protein Precursors / metabolism
Sarcoma Viruses, Murine / metabolism*
Serine / metabolism
Viral Proteins / biosynthesis*,  metabolism
Chemical
Reg. No./Substance:
0/Phosphoproteins; 0/Protein Precursors; 0/Viral Proteins; 56-45-1/Serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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