| Detection of a transforming gene product in cells transformed by Moloney murine sarcoma virus. | |
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MedLine Citation:
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PMID: 6288258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We identified, in cells transformed by Moloney murine sarcoma virus (M-MuSV clone 124), a protein encoded by the M-MuSV transforming gene, v-mos. An antiserum against a synthetic peptide corresponding to the C terminus of a protein predicted from the v-mos nucleotide sequence specifically recognizes a protein doublet of approximately 37,000 daltons from 35S-methionine-labeled M-MuSV 124-transformed producer cells. By peptide mapping, this protein is almost identical to the 37 kd in vitro translation product from the M-MuSV v-mos gene. Immunoprecipitates from 32P-labeled cells contain a single v-mos-specific phosphoprotein, which has at least six sites of phosphorylation containing phosphoserine. Pulse-chase experiments show that the lower band in the 35S-methionine-labeled doublet is the primary translation product, which is modified, probably by phosphorylation, to yield the upper band. A similar mos protein is immunoprecipitated from HT1-MuSV-transformed cells, but not from uninfected NIH/3T3 cells. These mos proteins are present at very low levels in transformed cell lines. Cells acutely infected with M-MuSV 124, however, transiently contain much higher levels of the mos protein. These high levels coincide with extensive cell mortality. |
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Authors:
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J Papkoff; I M Verma; T Hunter |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell Volume: 29 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1982 Jun |
Date Detail:
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Created Date: 1982-12-02 Completed Date: 1982-12-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 417-26 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Transformation, Viral* Cells, Cultured Fibroblasts / metabolism Mice Moloney murine leukemia virus / metabolism* Phosphoproteins / biosynthesis Phosphorylation Protein Precursors / metabolism Sarcoma Viruses, Murine / metabolism* Serine / metabolism Viral Proteins / biosynthesis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Phosphoproteins; 0/Protein Precursors; 0/Viral Proteins; 56-45-1/Serine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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