Document Detail


Detection of staphylococcal enterotoxins in dairy products by the reversed passive latex agglutination (SET-RPLA) kit.
MedLine Citation:
PMID:  2641487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The SET-RPLA is a commercially available kit for the detection of staphyloccal enterotoxins in foods. Previous reports have shown that non-specific reactions occur on use of the kit with cheeses and thereby restrict its use. In this study a variety of dairy products were tested and non-specific reactions were found to be associated with those products rennetted during manufacture. These reactions can be obviated by addition of 10 mM sodium hexametaphosphate to the diluent provided in the kit, without affecting the ability to detect staphyloccal enterotoxins in dairy products. The sensitivity of the SET-RPLA was demonstrated to be 0.25 ng/ml.
Authors:
S A Rose; P Bankes; M F Stringer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  8     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1990-10-02     Completed Date:  1990-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-72     Citation Subset:  IM    
Affiliation:
Campden Food and Drink Research Association, Gloucestershire, U.K.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cheese / analysis
Dairy Products / analysis*
Enterotoxins / analysis*
Food Contamination / analysis*
Predictive Value of Tests
Reagent Kits, Diagnostic
Staphylococcus aureus*
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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