Document Detail


Detection of staphylococcal enterotoxin B in milk and milk products using immunodiagnostic lateral flow devices.
MedLine Citation:
PMID:  20480905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcal enterotoxin B (SEB) is an extracellular pyrotoxin produced by Staphylococcus aureus, a known etiologic agent of food poisoning in humans. Lateral flow immunochromatographic devices (LFDs) designed for the environmental detection of SEB were adapted for use in this study to detect SEB in milk containing 2% fat, chocolate-flavored milk, and milk-derived products such as yogurt, infant formula, and ice cream. The advantage of using LFDs in these particular food products was its ease and speed of use with no additional extraction methods needed. No false positives were observed with any of the products used in this study. Dilution of the samples overcame the Hook effect and permitted capillary flow into the membrane. Thus, semisolid products such as ice cream and some yogurts, and products containing thickeners needed to be diluted using a phosphate-buffered saline-based buffer, pH 7.2. SEB was easily detected at concentrations of 5 microg/mL and 500 ng/mL when the LFDs were used. SEB was also reliably detected at concentrations below 5 and 0.25 ng/mL, which may induce serious disease.
Authors:
Thomas Boyle; Joyce M Njoroge; Robert L Jones; Maryann Principato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-19     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  569-75     Citation Subset:  IM    
Affiliation:
U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 8301 Muirkirk Rd, Laurel, MD 20708, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chromatography / methods
Enterotoxins / analysis*
Equipment Design
False Positive Reactions
Food Analysis / instrumentation*,  methods
Food Contamination
Food Microbiology
Hydrogen-Ion Concentration
Ice Cream
Immunologic Techniques
Milk / metabolism*
Reproducibility of Results
Time Factors
Yogurt
Chemical
Reg. No./Substance:
0/Enterotoxins; 39424-53-8/enterotoxin B, staphylococcal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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