| Detection of stable N-terminal protachykinin A immunoreactivity in human plasma and cerebrospinal fluid. | |
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MedLine Citation:
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PMID: 18374454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Substance P (SP) is a neuropeptide that is released from sensory nerves and several types of immune cells. It is involved in the transmission of pain and has a number of pro-inflammatory effects. Like other neuropeptides, SP is derived from a large precursor peptide, protachykinin A (PTA). Alternative splicing results in the production of four distinct PTA molecules that all contain the sequence of SP and a common N-terminal region consisting of 37 amino acids. We have developed a sandwich immunoassay using antibodies against the N-terminal part of PTA. Here we demonstrate that N-terminal PTA immunoreactivity is present in human circulation and cerebrospinal fluid (CSF). The concentration was about 90 times higher in CSF than in EDTA-plasma. Analytical reversed phase HPLC revealed that NT-PTA 1-37 is the main immunoreactivity in human circulation and CSF. Moreover, compared to the low in vitro stability of SP of less than 12 min, NT-PTA immunoreactivity is absolutely stable in EDTA-plasma and CSF for more than 48 h. As NT-PTA 1-37 is produced in stoichiometric amounts and is theoretically co-released with SP, we suggest the measurement of NT-PTA immunoreactivity as surrogate molecule for the release of bioactive SP. |
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Authors:
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Andrea Ernst; Jennifer Suhr; Josef Köhrle; Andreas Bergmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-19 |
Journal Detail:
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Title: Peptides Volume: 29 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-09 Completed Date: 2008-09-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: United States |
Other Details:
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Languages: eng Pagination: 1201-6 Citation Subset: IM |
Affiliation:
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SphingoTec GmbH, Tulpenweg 6, D-16556 Borgsdorf, Germany. ernst310577@gmx.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Chelating Agents / metabolism Chromatography, High Pressure Liquid Drug Stability Edetic Acid / blood Humans Immunoassay / methods* Molecular Sequence Data Protein Precursors / blood*, cerebrospinal fluid*, chemistry, immunology* Tachykinins / blood*, cerebrospinal fluid*, chemistry, immunology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Protein Precursors; 0/Tachykinins; 0/protachykinin; 60-00-4/Edetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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