Document Detail


Detection and stability of the major almond allergen in foods.
MedLine Citation:
PMID:  11368566     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Almond major protein (AMP or amandin), the primary storage protein in almonds, is the major allergen recognized by almond-allergic patients. A rabbit antibody-based inhibition ELISA assay for detecting and quantifying AMP in commercial foods has been developed, and this assay, in conjunction with Western blotting analyses, has been applied to the investigation of the antigenic stability of AMP to harsh food-processing conditions. The ELISA assay detects purified AMP at levels as low as 87 +/-16 ng/mL and can detect almond at between 5 and 37 ppm in the tested foods. The assay was used to quantify AMP in aqueous extracts of various foods that were defatted and spiked with known amounts of purified AMP or almond flour. In addition, AMP was quantified in commercially prepared and processed almond-containing foods. Neither blanching, roasting, nor autoclaving of almonds markedly decreased the detectability of AMP in subsequent aqueous extracts of almonds. Western blots using both rabbit antisera and sera from human almond-allergic patients confirm a general stability of the various peptides that comprise this complex molecule and show that the rabbit antibody-based assay recognizes substantially the same set of peptides as does the IgE in sera from almond-allergic patients.
Authors:
K H Roux; S S Teuber; J M Robotham; S K Sathe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  49     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-12-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2131-6     Citation Subset:  IM    
Affiliation:
Department of Biological Science, Florida State University, Tallahassee, Florida 32306, USA. roux@bio.fsu.edu
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis*,  immunology
Animals
Antibody Formation
Blotting, Western
Enzyme-Linked Immunosorbent Assay / methods
Food Hypersensitivity / immunology*
Humans
Nuts / adverse effects,  chemistry,  immunology*
Plant Proteins / analysis*,  immunology
Rabbits
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Allergens; 0/Plant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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