| Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction. | |
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MedLine Citation:
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PMID: 20835581 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR). METHODOLOGY: Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine prevalence rates, which were then compared using a chi-square test. Significance level was set at p<0.05. Mean and standard deviation values were used to evaluate variations in prevalence according to site. RESULTS: The prevalence of S. mutans was 70% in saliva; 60% in samples collected from the tongue dorsum; 50% in samples collected from the buccal mucosa; 56.5% in the supragingival plaque; and 53.5% in the subgingival plaque. The prevalence of E. faecalis ranged from 3.5% to 13.5% in all intraoral microenvironment. The highest prevalence of P. gingivalis was found in subgingival plaque (53.5%), and of P. intermedia in supragingival plaque (33.5%), subgingival plaque (30%) and tongue dorsum (33.5%). The prevalence of bacteria did not vary significantly among the intraoral sites. CONCLUSIONS: All studied bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to be an adequate method for epidemiological studies. |
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Authors:
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Cyntia Rodrigues de Araújo Estrela; Fabiana Cristina Pimenta; Ana Helena Gonçalves de Alencar; Luis Fernando Naldi Ruiz; Carlos Estrela |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied oral science : revista FOB Volume: 18 ISSN: 1678-7765 ISO Abbreviation: J Appl Oral Sci Publication Date: 2010 Jul-Aug |
Date Detail:
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Created Date: 2010-09-13 Completed Date: 2010-12-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101189774 Medline TA: J Appl Oral Sci Country: Brazil |
Other Details:
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Languages: eng Pagination: 426-31 Citation Subset: D; IM |
Affiliation:
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Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bacteroidaceae / classification* Cheek / microbiology Chronic Periodontitis / classification, microbiology* Dental Plaque / microbiology Enterococcus faecalis / isolation & purification Female Gingiva / microbiology Gingival Hemorrhage / classification Humans Lactobacillales / classification* Male Mouth Mucosa / microbiology Periodontal Pocket / classification Polymerase Chain Reaction / methods* Porphyromonas gingivalis / isolation & purification Prevotella intermedia / isolation & purification Saliva / microbiology Streptococcus mutans / isolation & purification Tongue / microbiology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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