Document Detail


Detection of rubella virus-specific immunoglobulin G in saliva by an amplification-based enzyme-linked immunosorbent assay using monoclonal antibody to fluorescein isothiocyanate.
MedLine Citation:
PMID:  9889225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunoglobulin G (IgG)-capture enzyme-linked immunosorbent assay (ELISA) for rubella virus is described. The assay uses a fluorescein isothiocyanate (FITC)-anti-FITC amplification system. The detection limit of the ELISA was approximately 7 IU of rubella virus-specific IgG per ml of serum sample. For saliva samples the performances of the capture ELISA and previously described radioimmunoassay were assessed, and the results of those two assays were compared to the rubella virus-specific IgG result obtained by a commercial ELISA (Behring Enzygnost) with a panel of paired serum and saliva samples. This comparison showed that the capture ELISA with saliva was more sensitive than the radioimmunoassay and that the results correlated better with the serum IgG result than the results of the radioimmunoassay did, with an overall sensitivity of 82% and a rank correlation of 0.68, whereas the sensitivity and rank correlation for the radioimmunoassay were 74% and 0.45, respectively. For subjects of 10 years of age or younger, the ELISA with saliva had a sensitivity of 94% and a specificity of 100% compared to the results of the ELISA (Behring Enzygnost) for rubella virus-specific IgG with corresponding serum samples. The sensitivity was much lower for subjects ages 17 years or older. The assay may have wider epidemiological use with saliva specimens, particularly those from children.
Authors:
A J Vyse; D W Brown; B J Cohen; R Samuel; D J Nokes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  37     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-02-24     Completed Date:  1999-02-24     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  391-5     Citation Subset:  IM    
Affiliation:
Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom. avyse@phis.co.uk
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Antibodies, Monoclonal / immunology
Antibodies, Viral / analysis*,  blood
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Fluorescein-5-isothiocyanate
Humans
Immunoglobulin G / analysis*,  blood
Infant
Predictive Value of Tests
Radioimmunoassay
Rubella / epidemiology*
Rubella virus / immunology*
Saliva / immunology*
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Immunoglobulin G; 0/rubella antibodies; 3326-32-7/Fluorescein-5-isothiocyanate
Comments/Corrections

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