| Detection of repair activity during the DNA damage-induced G2 delay in human cancer cells. | |
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MedLine Citation:
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PMID: 11429695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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All eukaryotic cells manifest cell cycle delay after exposure to DNA damaging agents. It has been proposed that such cell cycle checkpoints may allow DNA repair but direct evidence of such activity during the radiation-induced G2 delay has been lacking. We report here that cells arrested in G2 by radiation (2-3 Gy) and etoposide incorporate bromodeoxyuridine (BrdU) at discrete foci in the nucleus. We detected G2 cells with CENP-F, a nuclear protein maximally expressed in G2. Caffeine and okadaic acid, both established radiosensitizers, inhibit the incorporation of BrdU in G2 cells. Radioresistant HT29 and OVCAR cells demonstrate BrdU foci formation more frequently during the G2 delay when compared to the more radiosensitive A2780 cell line. The repair foci formed during G2 may be followed through mitosis and observed in daughter cells in G1. Taken together, these observations are consistent with the detection of DNA repair activity during the radiation-induced G2 delay after relatively low doses of radiation. |
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Authors:
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G D Kao; W G McKenna; T J Yen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Oncogene Volume: 20 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-06-28 Completed Date: 2001-07-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
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Languages: eng Pagination: 3486-96 Citation Subset: IM |
Affiliation:
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Hospital of the University of Pennsylvania, Department of Radiation Oncology, 2 Donner, 3400 Spruce Street, Philadelphia, Pennsylvania, PA 19104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bromodeoxyuridine Cell Cycle / drug effects, genetics*, radiation effects Centromere / genetics Chromosomal Proteins, Non-Histone / genetics DNA Damage* DNA Repair* DNA, Neoplasm / drug effects, genetics*, radiation effects Etoposide / toxicity Female Flow Cytometry G2 Phase Gamma Rays* Hela Cells Humans Kinetics Microfilament Proteins Ovarian Neoplasms Radiation Tolerance Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA06927/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromosomal Proteins, Non-Histone; 0/DNA, Neoplasm; 0/Microfilament Proteins; 0/centromere protein F; 33419-42-0/Etoposide; 59-14-3/Bromodeoxyuridine |
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