Document Detail

Detection of repair activity during the DNA damage-induced G2 delay in human cancer cells.
MedLine Citation:
PMID:  11429695     Owner:  NLM     Status:  MEDLINE    
All eukaryotic cells manifest cell cycle delay after exposure to DNA damaging agents. It has been proposed that such cell cycle checkpoints may allow DNA repair but direct evidence of such activity during the radiation-induced G2 delay has been lacking. We report here that cells arrested in G2 by radiation (2-3 Gy) and etoposide incorporate bromodeoxyuridine (BrdU) at discrete foci in the nucleus. We detected G2 cells with CENP-F, a nuclear protein maximally expressed in G2. Caffeine and okadaic acid, both established radiosensitizers, inhibit the incorporation of BrdU in G2 cells. Radioresistant HT29 and OVCAR cells demonstrate BrdU foci formation more frequently during the G2 delay when compared to the more radiosensitive A2780 cell line. The repair foci formed during G2 may be followed through mitosis and observed in daughter cells in G1. Taken together, these observations are consistent with the detection of DNA repair activity during the radiation-induced G2 delay after relatively low doses of radiation.
G D Kao; W G McKenna; T J Yen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  20     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-28     Completed Date:  2001-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  3486-96     Citation Subset:  IM    
Hospital of the University of Pennsylvania, Department of Radiation Oncology, 2 Donner, 3400 Spruce Street, Philadelphia, Pennsylvania, PA 19104, USA.
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MeSH Terms
Cell Cycle / drug effects,  genetics*,  radiation effects
Centromere / genetics
Chromosomal Proteins, Non-Histone / genetics
DNA Damage*
DNA Repair*
DNA, Neoplasm / drug effects,  genetics*,  radiation effects
Etoposide / toxicity
Flow Cytometry
G2 Phase
Gamma Rays*
Hela Cells
Microfilament Proteins
Ovarian Neoplasms
Radiation Tolerance
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/DNA, Neoplasm; 0/Microfilament Proteins; 0/centromere protein F; 33419-42-0/Etoposide; 59-14-3/Bromodeoxyuridine

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