Document Detail

Detection of repair activity during the DNA damage-induced G2 delay in human cancer cells.
MedLine Citation:
PMID:  11429695     Owner:  NLM     Status:  MEDLINE    
All eukaryotic cells manifest cell cycle delay after exposure to DNA damaging agents. It has been proposed that such cell cycle checkpoints may allow DNA repair but direct evidence of such activity during the radiation-induced G2 delay has been lacking. We report here that cells arrested in G2 by radiation (2-3 Gy) and etoposide incorporate bromodeoxyuridine (BrdU) at discrete foci in the nucleus. We detected G2 cells with CENP-F, a nuclear protein maximally expressed in G2. Caffeine and okadaic acid, both established radiosensitizers, inhibit the incorporation of BrdU in G2 cells. Radioresistant HT29 and OVCAR cells demonstrate BrdU foci formation more frequently during the G2 delay when compared to the more radiosensitive A2780 cell line. The repair foci formed during G2 may be followed through mitosis and observed in daughter cells in G1. Taken together, these observations are consistent with the detection of DNA repair activity during the radiation-induced G2 delay after relatively low doses of radiation.
G D Kao; W G McKenna; T J Yen
Related Documents :
8764865 - Cell cycle analysis of proliferative zone chondrocytes in growth plates elongating at d...
7388975 - Biochemical controls of the g1 phase of a mammalian cell cycle. i. analysis of chromati...
20724915 - Clotrimazole induces a late g1 cell cycle arrest and sensitizes glioblastoma cells to r...
15056365 - Synchronization of plodia interpunctella lepidopteran cells and effects of 20-hydroxyec...
2998945 - A shuttle vector plasmid for studying carcinogen-induced point mutations in mammalian c...
1900065 - Differential adherence of hydrophobic and hydrophilic candida albicans yeast cells to m...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  20     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-28     Completed Date:  2001-07-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  3486-96     Citation Subset:  IM    
Hospital of the University of Pennsylvania, Department of Radiation Oncology, 2 Donner, 3400 Spruce Street, Philadelphia, Pennsylvania, PA 19104, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle / drug effects,  genetics*,  radiation effects
Centromere / genetics
Chromosomal Proteins, Non-Histone / genetics
DNA Damage*
DNA Repair*
DNA, Neoplasm / drug effects,  genetics*,  radiation effects
Etoposide / toxicity
Flow Cytometry
G2 Phase
Gamma Rays*
Hela Cells
Microfilament Proteins
Ovarian Neoplasms
Radiation Tolerance
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/DNA, Neoplasm; 0/Microfilament Proteins; 0/centromere protein F; 33419-42-0/Etoposide; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Key role of Shc signaling in the transforming pathway triggered by Ret/ptc2 oncoprotein.
Next Document:  Downregulation of the potential suppressor gene IGFBP-rP1 in human breast cancer is associated with ...